文章摘要
杨 健;郭增旺;刁静静;马 萍;李朝阳;于 笛;王凯凯;张丽萍.绿豆肽对RAW264.7巨噬细胞增殖及免疫活性物质的影响[J].中国食品学报,2019,19(8):22-30
绿豆肽对RAW264.7巨噬细胞增殖及免疫活性物质的影响
  
DOI:
中文关键词: 绿豆肽  巨噬细胞  细胞增殖  免疫活性物质
英文关键词: mung bean petides  macrophages  cell proliferation  immunologically active substances
基金项目:国家自然科学基金项目(31471586)
作者单位
杨 健;郭增旺;刁静静;马 萍;李朝阳;于 笛;王凯凯;张丽萍 国家杂粮工程技术研究中心黑龙江大庆163319黑龙江八一农垦大学黑龙江大庆163319 
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中文摘要:
      研究不同浓度绿豆肽(MBPs)对RAW264.7巨噬细胞增殖、糖原和核酸含量的影响,对酸性ATP酶、溶菌酶(LZM)和超氧化物歧化酶(SOD)的活性及细胞因子的影响。以不同浓度的MBPs单独刺激和不同浓度的MBPs和LPS共同刺激RAW264.7巨噬细胞,结果显示:MBPs能够促进RAW264.7巨噬细胞的增殖;细胞内糖原染色、核内DNA染色及胞质内RNA染色加深,细胞活性增强;MBPs可以显著促进RAW264.7巨噬细胞内ATPase的活力,增强LZM、SOD活性及促炎性细胞因子的分泌水平;MPBs能够缓解LPS对巨噬细胞增殖及酸性ATP酶、溶菌酶(LZM)和超氧化物歧化酶(SOD)活性的抑制作用,下调LPS诱导的促炎性细胞因子的表达。
英文摘要:
      The effects of different concentrations of mung bean petides (MBPs) on RAW264.7 macrophage proliferation, glycogen, nucleic acid content, acid and enzyme effects, lysozyme (LZM), superoxide dismutase(SOD) and cytokines were studied. Use MBPs individually or in different concentration of different concentrations of MBPs and LPS jointly handle RAW264.7 macrophages. The results show that the MBPs can promote the proliferation of RAW264.7 macrophages, intracellular glycogen dyeing and nuclear DNA and cytoplasmic RNA in dyeing. MBPs can significantly promote the ATPase activity, significantly enhance LZM, SOD activity and cytokine secretion in RAW264.7 macrophages. The index above decreased caused by LPS overstimulation can be return to nearly normal by using MBPs. The MPBs can alleviate the inhibitory effect of LPS on macrophage proliferation, acidase ATPase, lysozyme (LZM) and superoxide dismutase (SOD) activity, and down-regulate the expression of LPS-induced proinflammatory cytokines.
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