文章摘要
茹 毅;沈宁燕;倪 辉;陈艳红;肖安风.乙醇促进法夫酵母虾青素合成的机理及其代谢调控[J].中国食品学报,2019,19(8):41-48
乙醇促进法夫酵母虾青素合成的机理及其代谢调控
  
DOI:
中文关键词: 法夫酵母  乙醇  虾青素合成  代谢通量分析  代谢调控
英文关键词: Phaffia rhodozyma  ethanol  astaxanthin synthesis  metabolic flux analysis  metabolic regulation
基金项目:国家自然科学基金项目(31501448);福建省教育厅项目(JA15266);厦门南方海洋研究中心项目(14CZP035HJ09)
作者单位
茹 毅;沈宁燕;倪 辉;陈艳红;肖安风 集美大学食品与生物工程学院福建厦门361021福建省食品微生物与酶工程重点实验室福建厦门361021福建省海洋功能食品工程技术研究中心福建厦门361021厦门市海洋功能食品重点实验室福建厦门361021 
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中文摘要:
      采用代谢通量分析的方法,分析乙醇促进法夫酵母生物合成虾青素的代谢调控机理。研究发现,乙醇促进法夫酵母细胞代谢中丙酮酸、乙酰辅酶A代谢节点处的通量,使流向虾青素合成途径的通量增加,是对照组通量的2.3倍,进而促进了虾青素的合成。根据代谢调控机理,对法夫酵母菌株JMU-MVP14合成虾青素代谢途径的α-酮戊二酸和5-磷酸核酮糖代谢节点进行调控,结果表明:在培养环境中添加0.5 g/L α-酮戊二酸,能促进法夫酵母细胞生长,生长量提高0.4 g/L。添加3 g/L的谷氨酸时,法夫酵母总类胡萝卜素产量达67.9 mg/L,是对照试验总类胡萝卜素产量的1.7倍。
英文摘要:
      Metabolic flux analysis was used to analyze the metabolic regulation mechanism of ethanol to promote the biosynthesis of astaxanthin. It was found that ethanol promoted the flux of pyruvate and acetyl-CoA metabolic nodules in the cell metabolism of Phaffia rhodozyma, and the flux of astaxanthin synthesis pathway was increased by 2.3 times of that of the control group, which promoted the synthesis of astaxanthin. According to the metabolic regulation mechanism, the metabolizing nodes of α-keto-glutaric acid and 5-phosphate ribulose were studied involved by the astaxanthin synthesis pathway of Phaffia rhodozyma JMU-MVP14. It was found that addition of 0.5 g/L α-keto-glutaric acid into culture broth could promote cell growth of Phaffia rhodozyma by 0.4 g/L. And astaxanthin yield reached 67.9 mg/L when 3 g/L glutamic acid was added into the broth, which was 1.7 times that of the control.
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