文章摘要
林 洋;崔天琦;吕欣然;白凤翎;励建荣;沈 琳.传统发酵蔬菜中抑制嗜水气单胞菌群体感应及生物膜形成乳酸菌的筛选与鉴定[J].中国食品学报,2019,19(8):199-207
传统发酵蔬菜中抑制嗜水气单胞菌群体感应及生物膜形成乳酸菌的筛选与鉴定
  
DOI:
中文关键词: 乳酸菌  抑制  嗜水气单胞菌  群体感应  生物膜
英文关键词: lactic acid bacteria  inhibit  Aeromonas hydrophila  quorum sensing  biofilm
基金项目:名优水产品品质溯源技术及监管体系研究与示范项目(2015BAD17B05)
作者单位
林 洋;崔天琦;吕欣然;白凤翎;励建荣;沈 琳 渤海大学食品科学与工程学院辽宁省食品安全重点实验室生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁锦州121013北京林业大学生物科学与技术学院北京100083大连东霖食品股份有限公司辽宁大连116101 
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中文摘要:
      目的:从来源于传统发酵蔬菜的乳酸菌中筛选对嗜水气单胞菌群体感应及生物膜形成有抑制作用的菌株。方法:采用牛津杯打孔法筛选乳酸菌菌株,测定其对N酰基高丝氨酸内酯(AHLs)信号分子的降解率。利用96孔板法测定其对生物膜的抑制率,应用光镜和扫描电镜观察其对生物膜形成的影响。最终通过生理生化试验和16S rRNA序列分析鉴定乳酸菌菌株。结果:分离自安徽绩溪酸豆角的菌株AJS2-4对嗜水气单胞菌群体感应有抑制作用,当其代谢产物粗提物质量浓度为4 mg/mL时,对嗜水气单胞菌AHLs的降解率为49.36%,对其生物膜抑制率为32.25%;当其质量浓度达到8 mg/mL时,可使嗜水气单胞菌的AHLs完全降解。该粗提物经 121 ℃处理30 min活性基本不变,蛋白酶处理使其活性完全丧失,可初步判定菌株AJS2-4粗提物中的抑制群体感应活性成分为蛋白类物质,且具有热稳定性。光镜结果显示:菌株AJS2-4粗提物可有效抑制嗜水气单胞菌生物膜的合成。扫描电镜结果显示:菌株AJS2-4粗提物不仅能有效降低其生物膜的生成量,还可使其结构破裂变得疏松。经生理生化和16S rRNA鉴定菌株AJS2-4为植物乳杆菌(Lactobacillus plantarum)。结论:从安徽绩溪酸豆角中获得1株抑制嗜水气单胞菌群体感应和生物膜形成的植物乳杆菌 AJS2-4。
英文摘要:
      Objective: To screen lactic acid bacteria (LAB) with inhibitory activity against quorum sensing and biofilm formation of Aeromonas hydrophila from traditional fermented vegetables. Methods: Screening and degradation N-acyl-homoserine lactones (AHLs) of LAB was measured using Oxford cup agar well diffusion method, the inhibitory rate of biofilm was determined using the 96-well microtiter plates. The biofilm formation of A. hydrophila was observed by light microscopy and scanning electron microscope(SEM). Strain AJS2-4 was identified by biochemical tests and 16S rRNA sequence analysis. Results: Strain AJS2-4 with inhibitory activity toward quorum sensing of A. hydrophila was isolated from acid beans in Jixi, Anhui Province. 4 mg/mL crude extraction from strain AJS2-4 degraded the AHLs of A. hydrophila and degradation rate reached 49.36%, inhibited the biofilm formation of A. hydrophila and inhibitory rate reached 32.25%. 8 mg/mL crude extraction from strain AJS2-4 completely degraded the AHLs of A. hydrophila. The crude extraction from strain AJS2-4 remained inhibition activity after treatment at 121 ℃ for 30 min. However, its activity completely lost after treatment with protease. Therefore, the inhibition ingredient of crude extraction from strain AJS2-4 was preliminarily determined as proteins with heat-stable. Light microscopy revealed that crude extraction from strain AJS2-4 inhibited biofilm formation of A. hydrophila. The SEM images also exhibited that crude extraction from strain AJS2-4 not only decreased the production of biofilm of A. hydrophila, but also loosed the structure of biofilm. Strain AJS2-4 was identified as Lactobacillus plantarum by physiology and biochemical test and 16S rRNA sequence analysis. Conclusion: Lb. plantarum AJS2-4 with inhibitory activity toward quorum sensing and biofilm formation of A. hydrophila was isolated from acid beans in Jixi, Anhui Province.
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