Impaired intestinal barrier commonly occurs during the renal dysfunction. In this study， we assessed the effect of probiotics on impaired intestinal barrier and related inflammation in 5/6 nephrectomy rat model （CKD rats）. The results showed that CKD rats impaired intestinal barrier was significantly imporved by gavage administration of 6×1010 CFU/d of probiotics mixture for ten weeks. treated by probiotics mixture product had remarkably lower lipopolysaccharide （LPS） in serum （from 0.19 EU/mL to 0.10 EU/mL， P<0.05）. This indicated that probiotics mixture reduced intestinal permeability. Taking probiotics mixture significantly increased crypt depth， thickness of mucosa and the number of goblet cells（P<0.05） in intestinal epithelium. Administration of probiotics mixture also increase expression of of ZO-1 and claudin-1（P<0.05） indicating the improvement of tight junction. Furthermore， Taking probiotics mixture significantly decreased the level of C-reaction protein and TNF-α （P<0.05）， and increased the level of IL-10 and IL-13 （P<0.05） in serum indicating balanced proinflammatory and anti-inflammatory reponses of host.