灰树花中改善脂质代谢活性组分的筛选及其提取工艺优化
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福建省自然科学基金面上项目(2016J01095)


Screening of Components for Improving Lipid Metabolism in Grifola frondosa and Optimization of Its Extraction Technology
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    目的:筛选灰树花中具有改善脂质代谢的活性组分并获得其最佳提取工艺条件。方法:采用模拟胆汁胶束法和脂肪酶抑制法两种体外筛选方法筛选灰树花中具有改善脂质代谢的活性组分,采用单因素以及响应面法优化灰树花活性组分的提取工艺。结果:灰树花乙酸乙酯提取物(EAE)具有较强的改善脂质代谢的活性,其对脱氧胆酸钠和胆酸钠的结合量最高,抑制脂肪酶活性最强。最佳提取工艺条件:料液比1∶29(g/mL)、超声时间30 min、提取温度50℃。通过超高效液相色谱-串联四极杆飞行时间质谱仪(UPLC-QTOF-MS/MS)对EAE组分进行鉴定,其主要成分为野靛黄素和矢车菊素-3-O-芸香糖苷。

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    Objective: The objective of this study was to screen out the active components with improved lipid metabolism in Grifola frondosa and to obtain the optimum extraction conditions. Method: Two kinds of in vitro screening methods, namely simulated bile micellar and lipase inhibition, were used to screen out the active components with stronger improving activity for lipid metabolism in Grifola frondosa. The extraction process of Grifola frondosa active components was optimized by single factor and response surface methodology. Result: The ethyl acetate extract (EAE) of Grifola frondosa had the strongest binding capacity of sodium deoxycholate and sodium cholate and inhibition of lipase activity. The best extraction conditions of the process were as follows: the ratio of solid to liquid was 1 ∶ 29(g/mL), the ultrasonic time was 30 min and the extraction temperature was 50 ℃. The major components of EAE were identified by UPLC-MS/MS and found to be isocetin and cyanidin-3-O-rutinoside.

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周文斌;洪家丽;李路;李燕;黄梓芮;郭伟灵;倪莉;饶平凡;刘斌;吕旭聪;.灰树花中改善脂质代谢活性组分的筛选及其提取工艺优化[J].中国食品学报,2020,20(2):145-153

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  • 在线发布日期: 2020-03-07
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