文章摘要
胡盛本;蔡澄亮;卓成飞;周若琳;陶林;邓泽元;李静;.银离子固相萃取法测定反刍动物反式脂肪酸异构体含量[J].中国食品学报,2020,20(2):280-288
银离子固相萃取法测定反刍动物反式脂肪酸异构体含量
Determination of Trans Fatty Acid Isomers in Ruminant Animals by Silver Ion Solid Phase Extraction Column
  
DOI:
中文关键词: 银离子固相萃取  反刍动物反式脂肪酸
英文关键词: silver ion solid phase extraction  ruminant trans fatty acid
基金项目:国家自然科学基金地区科学基金项目(316600447);江西省教育厅项目(GJJ150186)
作者单位
胡盛本;蔡澄亮;卓成飞;周若琳;陶林;邓泽元;李静; 南昌大学食品科学与技术国家重点实验室
 
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中文摘要:
      利用氨丙基硅胶柱从反刍动物脂质中分离甘油三酯和磷脂,以氢化大豆油作为对照。采用银离子固相萃取柱分离甘油三酯和磷脂中反式脂肪酸异构体,气相色谱仪测定反式脂肪酸含量。结果显示:在3种反刍动物脂质中检测出trans-16:1,trans-18:1和trans18:2 3类反式脂肪酸,其中总脂质和甘油三酯总反式脂肪酸含量最高的是羊肉脂质,分别占总脂肪酸的7.64%和7.38%;磷脂中总反式脂肪酸最高的是牛肉脂质,占总脂肪酸的5.89%。银离子固相萃取柱能够从反刍动物脂质中分离出9种trans18:1异构体,其中含量最高的是11t18:1,分别为总脂质、甘油三酯和磷脂trans18:1的30.33%~39.03%,38.08%~45.09%和27.10%~44.47%。通过PLS-DA分析3种反刍动物反式脂肪酸异构体之间的差异,羊肉脂质中甘油三酯中的9t18:1和磷脂中的11t18:1含量均显著高于牛奶脂质和牛肉脂质(P<0.05);牛肉脂质中磷脂中的9t18:1和15t18:1含量均显著高于牛奶脂质和羊肉脂质(P<0.05);牛奶脂质磷脂中的12t18:1和16t18:1的含量均显著高于牛肉脂质和羊肉脂质(P<0.05)。反刍动物磷脂中的反式脂肪酸异构体有较大差异。
英文摘要:
      NH2 solid phase extraction cartridges were used to separate triglycerides and phospholipids from ruminant fatty acids and hydrogenated soybean oil and partially hydronated soybean oil were used as control groups. Then silver ion solid phase extraction columns were applied to elute trans octadecenoic acid isomers from triglycerides and phospholipids before detected by gas chromatography. Results showed three kinds of trans fatty acids were found in ruminant animals lipids and sheep lipids has higher trans fatty acids in total lipids and triglycerides with 7.64% and 7.38%, while beef fat has richest trans fatty acids in phospholipids with 5.89%. In addition, 9 types trans octadecenoic acid isomers were separated and detected by gas chromatography, in which the 11t18:1 was the richest isomer in ruminant animals lipids by the content of 30.33%-39.03% in trans octadecenoic acids of total lipids, 38.08%-45.09% in triglycerides and 27.10%-44.47% in phospholipids of ruminant animal lipids. PLS-DA analysis showed difference among three ruminant animals on trans fatty acids isomers, sheep lipids had higher 9t18:1 in triglycerides and 11t18:1 in phospholipids, beef lipids had richer 9t18:1 and 15t18:1 in phospholipids and milk fat had higher 12t18:1 and 16t18:1 in phospholipids. Thus phospholipids varied in ruminant animal lipids.
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