文章摘要
梁秋芳;张熙;任晓锋;侯婷;瞿志超;冯洛;马海乐;.扫频超声处理时间对β-乳球蛋白酶解制备多肽抗氧化活性的影响[J].中国食品学报,2020,20(4):33-41
扫频超声处理时间对β-乳球蛋白酶解制备多肽抗氧化活性的影响
Effect of Sweeping Frequency Ultrasound Pretreatment Time on the Antioxidant Activity of Peptides Prepared by β-Lactoglobulin
  
DOI:
中文关键词: 扫频超声  β-乳球蛋白  抗氧化活性  分子质量  疏水性  氨基酸组成
英文关键词: sweeping frequency ultrasound  β-lactoglobulin  antioxidant activity  molecular weight distribution  surface hydrophobicity  the composition of amino acids
基金项目:国家自然科学基金项目(31771977); 江苏高校优势学科建设工程资助项目; 江苏省博士后基金项目(2019K114); 江苏省农产品物理加工重点实验室开放基金(JAPP2014-3)
作者单位
梁秋芳;张熙;任晓锋;侯婷;瞿志超;冯洛;马海乐; 江苏大学食品与生物工程学院
江苏大学食品物理加工研究院
 
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中文摘要:
      目的:研究不同扫频超声处理时间对β-乳球蛋白酶解制备多肽抗氧化活性的影响。方法:研究不同的超声预处理时间(10,20,30,60,90 min)对β-乳球蛋白表观结构的影响,以及超声波处理对β-乳球蛋白酶解产物的抗氧化活性、氨基酸组成、分子质量分布和疏水性的影响。结论:超声波处理可显著提高β-乳球蛋白酶解产物的DPPH自由基清除率、ABTS·清除能力和Fe2+络合能力。随着超声时间的延长,β-乳球蛋白酶解产物抗氧化活性呈先增加后降低的趋势。扫频超声波处理可以提高β-LG酶解产物的疏水性,并且显著增加多肽中的疏水性氨基酸的含量。扫频超声处理10~60 min有利于分子质量为200~2 000 u多肽的生成,从而提高其酶解产物的抗氧化活性。粒径分布表明短时间的超声处理(< 30 min)引起β-LG粒径减小,大分子蛋白的结构疏松,分子间的疏水性作用力增加;长时间(60~90 min)的超声处理则引起大分子蛋白聚集,疏水性作用力降低,蛋白颗粒粒径增大。
英文摘要:
      The aim of this study was to investigate the effect of sweeping frequency ultrasound pretreatment duration on the antioxidant activity of peptides prepared by β-lactoglobulin. The ultrasound pretreatment of different duration (0, 10, 20, 30, 60, 90 min) impacts on β-lactoglobulin (β-LG) hydrolysis and structural characteristics. The enzymatic hydrolysis characteristics of β-LG, such as the antioxidant activity, the surface hydrophobicity, the molecular weight distribution and the composition of amino acids of β-lactoglobulin hydrolysates were studied. The particle size, surface hydrophobicity and microstructure of β-LG after sweeping frequency ultrasound pretreatment were also determined. The results showed that sweeping frequency ultrasound pretreatment significantly improved the DPPH· scavenging rate, ABTS· scavenging rate and Fe2+ complexation rate. The antioxidant activity of β-LG hydrolysates increased significantly (P<0.05) with short time ultrasound pretreatment and decreased over a longer period (60,90 min). After sweeping frequency ultrasound pretreatments, the surface hydrophobicity of enzymatic hydrolysates increased which indicated that more hydrophobic substances were released and polypeptides with molecular weight 200-2 000 u increased significantly which improved the antioxidant activity of β-LG hydrolysates. The amino acid profile analysis indicated sweeping frequency ultrasound pretreatment could increase the concentration of hydrophobic amino acids of the β-LG hydrolysates. Short time (< 30 min) ultrasound induced the particle size largely increased. After long-time sweeping frequency ultrasound (60, 90 min) treatment, the particle size of β-LG increased, which indicated that the exposed tryptophan residues aggregated into the protein.
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