文章摘要
陈百莹;郑苗;邓泽元;李红艳.植物乳杆菌ZJ316培养基优化和高密度培养的研究[J].中国食品学报,2020,20(7):65-74
植物乳杆菌ZJ316培养基优化和高密度培养的研究
Optimization of Fermentation Medium and the High-density Culturing Conditions for Lactobacillus plantarum ZJ316
  
DOI:
中文关键词: 植物乳杆菌  响应面  高密度培养  补料分批培养
英文关键词: Lactobacillus plantarum  response surface  high-density culture  fed batch fermentation
基金项目:江西省杰出青年人才资助计划项目(20162BCB23018)
作者单位
陈百莹;郑苗;邓泽元;李红艳 南昌大学食品科学与技术国家重点实验室 
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中文摘要:
      针对植物乳杆菌ZJ316,通过单因素和响应面试验设计法,对其培养基配方进行优化。同时,对植物乳杆菌ZJ316高密度培养条件、菌体收集条件和分批补料方法进行研究。结果表明,植物乳杆菌ZJ316优化培养基(质量分数)为:葡萄糖2.71%,酵母粉0.45%,牛肉膏0.6%。最佳培养条件为:培养基初始pH 6,接种量4%,摇床转速80 r/min,培养温度35 ℃。在离心条件为4 000 r/min,20 min时,植物乳杆菌菌体成活率达到最大值为81.09%。此外,采用间歇补料的方法,为增大菌体数可适当添加氮源,菌体数可达9.28×109 CFU/mL。
英文摘要:
      The condition of Lactobacillus plantarum formulation was optimized by the single factor and response surface methodology. Meanwhile, the conditions of high-density cultivation, collection of cells and fed batch fermentation method were studied. The results showed that the optimized medium was 2.71% of glucose, yeast 0.45% and beef extract 0.6%. The optimal cultivation conditions were as follows: initial pH 6, inoculation amount 4%, shaking speed 80 r/min, cultivation temperature 35 ℃. Under the conditions of 4 000 r/min and 20 min, Lactobacillus plantarum collection was at the highest survival rate, reaching 81.09%. In addition, the proper addition of nitrogen source can promote the proliferation of the cells, which could reached 9.28 × 109 CFU/mL.
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