文章摘要
迟海;李学英;徐春霞;杨宪时;鲁淑彦.产细菌素乳酸菌的筛选、鉴定及抑菌特性研究[J].中国食品学报,2020,20(7):181-187
产细菌素乳酸菌的筛选、鉴定及抑菌特性研究
Screening, Identification and Bacteriostatic Characteristics of Bacteriocin Producers from Lactic Acid Bacteria
  
DOI:
中文关键词: 细菌素  乳酸菌  筛选  广谱抑菌  食品致病菌
英文关键词: bacteriocin  lactic acid bacteria  screening  broad-inhibition spectrum  food-borne pathogens
基金项目:国家自然科学基金项目(31801663);上海市自然科学基金项目(18ZR1449900);中央级公益性科研院所基本科研业务费专项资金(2016M03);福建省属公益类科研院所基本科研专项(2018R1037-2)
作者单位
迟海;李学英;徐春霞;杨宪时;鲁淑彦 中国水产科学研究院东海水产研究所福建省闽东水产研究所上海海洋大学食品学院 
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中文摘要:
      从市售豆腐乳中筛选并获得4株具有抑制常见食品致病菌的乳酸菌。经分子、生理生化鉴定,这4株乳酸菌分别为乳酸乳球菌(DH2002和DH2004)、粪肠球菌(DH2001)和肠系膜明串珠菌(DH2003)。4株乳酸菌抑菌产物具有细菌素的一般特征(即耐高温和蛋白酶处理后失活),同时4株产细菌素乳酸菌对革兰氏阳性菌具有抑菌能力,主要可以抑制厚壁菌门的相关种属和常见的食品致病菌,对革兰氏阴性菌无抑制作用。分析乳酸菌细菌素初提物对金黄色葡萄球菌的抑制作用,结果表明:DH2001和DH2002可完全抑制/消除金黄色葡萄球菌,而DH2003在一定时间内可以阻止金黄色葡萄球菌生长。
英文摘要:
      In this study, a systemic screening for lactic acid bacteria with antimicrobial activity, from fermented Tofu against common food-borne pathogens was conducted. Four out of sixty-four isolates with antimicrobial activity against all the indicators (Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus) were obtained, and they were identified as Lactococcus lactis(named after DH2002 and DH2004), Enterococcus faecalis(named after DH2001) and Leuconostoc mesenteroides (named after DH2003) based on the results of 16S rDNA sequence and physiological and biochemical analysis. The bacteriocins produced by those four lactic acid bacteria had the typical bacteriocin traits, such as being of heat-stable and proteinase nature. Meanwhile, their inhibition spectrum contained many distantly related genera of Firmicutes, comprising most lactic acid bacteria as well as problematic species of Bacillus, Listeria, and Staphylococcus. However, no activity against Gram-negative bacteria was found. In addition, the model of actions of those bacteriocin producers was determined by using the partial purified bacteriocin against S. aureus. The results showed that DH2001 and DH2002 had bactericide activity, while DH2003 had bacteriostatic activity against S. aureus.
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