文章摘要
边燕飞;孙天松;孙志宏.微滴式数字PCR定量分析新生儿的肠道菌群[J].中国食品学报,2020,20(7):199-206
微滴式数字PCR定量分析新生儿的肠道菌群
The Quantitative Analysis of Neonatal Gut Microbiota by Droplet Digital PCR
  
DOI:
中文关键词: 新生儿  肠道菌群  分娩方式  生育年龄  微滴式数字PCR
英文关键词: neonate  gut microbiota  delivery mode  childbearing age  droplet digital PCR
基金项目:国家自然科学基金项目(31720103911,31622043);内蒙古自治区科技重大专项(zdzx2018018)
作者单位
边燕飞;孙天松;孙志宏 内蒙古农业大学乳品生物技术与工程教育部重点实验室农业部奶制品加工重点实验室 
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中文摘要:
      目的:研究表明新生儿出生时已携带大量细菌,而分娩方式是影响其早期肠道菌群建立的重要因素。目前多数研究只分析了不同分娩方式对新生儿肠道菌群结构的影响,而对新生儿肠道菌群的定量研究较少,且没有关于产妇生育年龄与新生儿肠道菌群的关联性研究。方法:采集14份新生儿胎便样品,利用微滴式数字PCR技术对胎便中的细菌总数以及核心菌群的肠杆菌科、乳杆菌属、双歧杆菌属、拟杆菌属、梭菌属、普氏菌属、链球菌属、乳球菌属、肠球菌属和大肠杆菌进行定量研究。结果:不同分娩方式对新生儿肠道细菌总数存在显著性差异(P<0.05),其中自然分娩新生儿肠道中肠杆菌科和乳杆菌属的绝对含量均显著高于剖腹产新生儿(P<0.05)。此外,高龄产妇所生新生儿肠道中链球菌属的绝对含量显著高于适龄产妇所生新生儿(P<0.05)。结论:产妇的分娩方式和生育年龄可能影响母婴菌群的传递。本研究采用的微滴式数字PCR技术具有高灵敏度,可用于新生儿肠道菌群的定量检测。
英文摘要:
      Objective: The researches show that newborns carry a large number of bacteria from the moment they were born. And delivery mode is an important factor influencing the establishment of the gut microbiota in infancy. However, most studies just analyzed the effect of different delivery mode on the structure of gut microbiota in newborns, the studies on quantification of neonatal gut microbiota are rare, and there is no report on the correlation between maternal childbearing age and neonatal gut microbiota. Methods: the neonatal meconium samples were studies from 14 infants, the total number of bacteria and core microbiota such as Enterobacteriaceae, Lactobacillus, Bifidobacterium, Bacteroides, Clostridium, Prevotella, Streptococcus, Lactococcus, Enterococcus and Escherichia coli in meconium were quantified using the droplet digital PCR. Results: There was a significant difference in the total number of bacteria present in the gut microbiota of infants with different delivery modes (P<0.05). The amounts of Enterobacteriaceae and Lactobacillus in the infants who were given birth naturally were significantly higher than those underwent caesarean section (P<0.05), indicating that delivery mode affects the transmission of microbes from mother to infant. In addition, the amounts of Streptococcus in the meconium of infant who given birth from the elder mother was significantly higher than those from the young mother (P<0.05). Conclusion: The maternal childbearing age and delivery mode affect the transmission of microbes from mother to infant. Droplet digital PCR technology has the advantage of high sensitivity, which can be used for quantitative detection of intestinal microbiota in newborn infants.
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