文章摘要
于笛;周伟;郭增旺;刁静静;迟治平;张丽萍.绿豆寡肽对脂多糖诱导巨噬细胞RAW264.7的抗炎作用[J].中国食品学报,2020,20(8):41-48
绿豆寡肽对脂多糖诱导巨噬细胞RAW264.7的抗炎作用
Anti-inflammatory Effect of Mung Bean Peptide on Lipopolysaccharide-induced
  
DOI:
中文关键词: 绿豆寡肽  RAW264.7细胞  脂多糖  炎症反应  抗炎活性
英文关键词: mungbeanpeptides  RAW264.7cells  lipopolyssacharide(LPS)  inflammatoryresponse  anti-inflammatoryactivity
基金项目:国家自然科学基金项目(31471586)
作者单位
于笛;周伟;郭增旺;刁静静;迟治平;张丽萍 黑龙江八一农垦大学国家杂粮工程技术研究中心 
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中文摘要:
      目的:将绿豆寡肽(MBPs)用于脂多糖( LPS)诱导巨噬细胞RAW264.7的炎症反应中,观察其对炎症是否有抑制作用。方法:用LPS构建细胞炎症模型,采用WST-1法检测细胞增殖能力,中性红法检测细胞吞噬能力,试剂盒法检测巨噬细胞髓过氧化物酶(MPO)、乳酸脱氢酶(LDH)活性及还原型谷胱甘肽(GSH)含量,ELISA法检测巨噬细胞细胞因子TNF-α、白介素-1α( IL-1α)、白介素-6(IL-6)的分泌量。结果:MBPs能显著缓解LPS对巨噬细胞增殖的抑制作用并下调其吞噬能力(P<0.05);MBPs能显著改善LPS诱导巨噬细胞MPO、LDH、GSH的水平(P<0.05);MBPs能显著抑制促炎性细胞因子TNF-αIL-1α、IL-6水平的上升。结论:MBPs通过调节巨噬细胞趋化吞噬能力、胞内酶解消化和降低促炎性细胞因子的水平达到缓解LPS诱导巨噬细胞炎症的作用,且呈现量效关系,具有一定的抗炎活性。
英文摘要:
      Objective: Mung bean peptides(MBPs) were used in lipopolysaccharide(LPS)-induced macrophage RAW264.7 inflammatory response to observe whether the inhibition of the inflammation produced. Methods: Using LPS to establish cell inflammation model. Using WST-1 method to detect cell proliferation, Neutral Red method to detect phagocytosis, KIT method to detect macrophage myeloperoxidase(MPO), lactate dehydrogenase(LDH) activity and reduced glutathione(GSH) content, ELISA to detect the levels of TNF-α, interleukin-1α(IL-1α) and interleukin-6(IL-6) in macrophages. Result: MBPs significantly attenuated the inhibitory effect of LPS on the proliferation of macrophages and down-regulated its phagocytosis(P<0.05). MBPs could significantly improve the level of MPO, LDH and GSH in macrophages induced by LPS(P<0.05). MBPs could significantly inhibit the increase of the levels of proinflammatory cytokines TNF-α, IL-1α, IL-6. Conclusion: MBPs could alleviate the LPS-induced macrophage inflammatory response by regulating the chemotactic phagocytosis of macrophages, intracellular digestion and digestion, and the decrease of proinflammatory cytokines levels, showing dose-response and anti-inflammatory activity.
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