文章摘要
付永岩;李盈盈;姜瞻梅;刘飞;姜成刚;侯俊财;田波;王玉堂.牛乳铁蛋白衍生肽FP-PF的安全性及其抑菌机理[J].中国食品学报,2020,20(8):92-99
牛乳铁蛋白衍生肽FP-PF的安全性及其抑菌机理
Safety and Antibacterial Mechanism of Bovine Lactoferrin Derived Peptide FP-PF
  
DOI:
中文关键词: 抗菌肽  溶血活性  细胞毒性  抑菌机理
英文关键词: antibacterialpeptide  hemolysisactivity  cytotoxicity  antibacterialmechanism
基金项目:“十三五”国家重点研发计划项目(2016YFD0400605)
作者单位
付永岩;李盈盈;姜瞻梅;刘飞;姜成刚;侯俊财;田波;王玉堂 东北农业大学乳品科学教育部重点试验室中国农业科学院哈尔滨兽医研究所 
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中文摘要:
      乳铁蛋白是乳中特有的一种新型抗菌、抗癌成分,具有广谱抗菌、消炎、抑制肿瘤细胞生长及调节机体免疫反应等作用。本试验以牛乳铁蛋白肽为基础,以对称结构理念设计的新抗菌肽FP-PF为试验对象,并以蜂毒素作为阳性对照肽,研究新型抗菌肽FP-PF的细胞毒性、溶血活性及抑菌机理。试验研究表明,在浓度1~256 μmol/L范围,FP-PF的溶血率都小于5%,表明其溶血率较低;细胞存活率大于92%,表细胞毒性较小。抑菌机理研究表明,抗菌肽FP-PF能破坏细胞外膜和内膜,改变细胞渗透压和完整性,从而达到杀菌和抑菌的效果。本试验设计的新型抗菌肽FP-PF有望在食品及化妆品等领域中应用。
英文摘要:
      Lactoferrin is a unique ingredient in milk, which has the functions of broad-spectrum antibacterial, anti-inflammatory, inhibition of tumor cell growth and regulation of immune response. It is a new antimicrobial and anticancer component. In this experiment, the new antimicrobial peptide FP-PF was designed with the idea of a symmetrical structure as the basis, and melittin was used as the positive control peptide. The cytotoxicity, hemolytic activity and bacteriostatic mechanism of the new antimicrobial peptides FP-PF were studied. The experimental results showed that the hemolysis rate of FP-PF which concentation was at the range of 1-256 mmol/L was less than 5%, indicating that the hemolysis rate was low. The cell survival rate was more than 92%, indicating that its cytotoxicity was small. Through the study of bacteriostatic mechanism, the antibacterial peptide FP-PF could damage the outer and inner membranes of cell membranes, change its osmotic pressure and integrity, and thus achieve the effect of sterilization and bacteriostasis. Therefore, the new antimicrobial peptide FP-PF designed in this experiment was expected to show its potential application value in food, cosmetics and so on.
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