文章摘要
刘慧琳;赵源;张瑛;唐佳琪;王静;.白酒和黄酒中生物胺的高效液相色谱分析法[J].中国食品学报,2020,20(8):248-254
白酒和黄酒中生物胺的高效液相色谱分析法
Determination of Biogenic Amines in Baijiu and Chinese Rice by High Performance Liquid Chromatography
  
DOI:
中文关键词: 黄酒  白酒  生物胺  高效液相色谱法
英文关键词: Chinese yellow wine  Baijiu  biogenic amines  high performance liquid chromatography (HPLC)
基金项目:国家重点研发计划项目(2018YFC1602300);北京市高创计划青年拔尖人才项目(2017000026833ZK28)
作者单位
刘慧琳;赵源;张瑛;唐佳琪;王静; 北京食品营养与人类健康高精尖创新中心北京市食品添加剂工程技术研究中心北京工商大学
 
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中文摘要:
      本研究建立了1种检测某白酒与黄酒中常见的9种生物胺(尸胺、组胺、酪胺、色胺、盐酸吡哆胺、精胺、亚精胺、腐胺和2-苯乙胺)的柱前衍生-高效液相色谱法,并对其实际运用可行性进行验证与讨论。以丹磺酰氯(Dns-Cl)作为衍生试剂,采用Thermo Hypersil GOLD C18色谱柱(250 mm×4.6 mm, 5μm)分离,以乙腈和含0.1%甲酸的0.01 mol/L乙酸铵水溶液作为流动相A与B,流速0.8 mL/min,紫外检测器波长254 nm。分析结果显示,9种生物胺在质量浓度0.5~50 mg/L范围,生物胺浓度与峰面积间线性相关性良好(R2>0.998)。选取1.0,5.0,10.0 mg/L 3个水平加标,得到加标回收率83.30%~114.7%,相对标准偏差均低于4.56%,9种生物胺检出限为0.07~0.22 mg/L。采用柱前衍生-高效液相色谱法分离、检测白酒与发酵酒——黄酒中的生物胺,实现了白酒与黄酒中生物胺含量的准确检测。
英文摘要:
      An analytical method for biogenic amines commonly(cadaverine, histamine, tyramine, tryptamine, pyridoxamine hydrochloride, spermine, spermidine, putrescine and 2-phenylethylamine) in Baijiu and Chinese rice wine by pre-column derivatization-HPLC was developed. The biogenic amines in Baijiu and Chinese rice wine were derived with dansyl chloride, separated by the C18 chromatographic column(250 mm×4.6 mm, 5 μm). The mixture of acetonitrile and water (0.01 mmol/L ammonium acetate, 0.1% formic acid) were served as the mobile phase at a flow rate of 0.8 mL/min and the ultraviolet (UV) detection wavelength was 254 nm. The results showed that in the concentration range of 0.5-50 mg/L, each of the 9 biogenic amine presented good linear correlation (R2 > 0.998). After the addition of a mixed standard solution of biogenic amines to Chinese yellow wine, the recovery rates were 83.30%-114.7%, with relative standard deviations (RSDs) below 4.56%, and the limit of detection for each component was 0.07-0.22 mg/L. Hence, the pre-column derivatization-HPLC method in this experiment could be used to separate and detect the biogenic amines in Baijiu and Chinese rice wine accurately.
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