富硒鼠李糖乳杆菌稳定性及其冻干保护剂研究
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陕西省科技厅农业科技计划项目(2017TSCXL-NY-02-03);陕西省教育厅专项科研计划项目(2018JK0121)


Studies on Stability of Selenium-enriched Lactobacillus rhamnosus and Its Lyophilized Protectant
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    摘要:

    采用电感耦合等离子发射光谱(ICP-OES)法测定富硒量和硒转化率,探索富硒能力较强的2株鼠李糖乳杆菌Lr-1和Lr-2在连续传250代过程中富硒能力的变化及其富硒稳定性。采用响应面法,优化富硒鼠李糖乳杆菌冻干保护剂配比,结果表明,鼠李糖乳杆菌在传250代过程中,菌株形态未发生明显变化。Lr-1平均富硒量为429.99 μg/g,平均硒转化率为28.32%;Lr-2平均富硒量为398.14 μg/g,平均硒转化率为25.57%,富硒能力较稳定。富硒鼠李糖乳杆菌最佳冻干保护剂配方为海藻糖7%、脱脂乳粉10%、谷氨酸钠5%,此条件下其冻干存活率达89.86%,比未加冻干保护剂时的冻干存活率提高81.85%。

    Abstract:

    The inductively coupled plasma emission spectroscopy (ICP-OES) method was used to determine the selenium-enriched amount and selenium conversion rate, and the two strains of Lactobacillus rhamnosus Lr-1 and Lr-2 with strong selenium-enriched ability were explored in the process of continuous passage for 250 generations. The selenium-enriched capacity was investigated to determine its selenium-enriched stability. On this basis, the response surface method was used to optimize the ratio of the selenium-enriched L. rhamnosus lyophilized protectant. The results showed that the morphology of the strain did not change significantly during the passage of 250 generations. The average selenium content of Lr-1 was 429.99 μg/g, and the average selenium conversion rate was 28.32%. The average selenium content of Lr-2 was 398.14 μg/g, the average selenium conversion rate was 25.57%, and the selenium-enriched ability was relatively stable. The best cryoprotectants for the selenium-enriched L. rhamnosus was 7% trehalose, 10% skim milk powder, 5% sodium glutamate, and its lyophilization survival rate was 89.86%, which was better than no cryoprotectants. The lyophilization survival rate was increased by 81.85%. This study provided a theoretical basis for the development of selenium-enriched lactic acid bacteria food.

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徐颖;贺黎;吕嘉枥;余海霞;王雪莹.富硒鼠李糖乳杆菌稳定性及其冻干保护剂研究[J].中国食品学报,2020,20(9):102-108

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  • 在线发布日期: 2020-10-13
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