This study established a DNA barcoding method for the identification of salmonids and then detected 17 commercial salmonid-derived products using this method. The gene fragments of DNA cytochrome C oxidase I （COI） and 16S ribosomal RNA （16S rRNA） were amplified from the samples for Sanger sequencing. The authenticity of the analyzed samples were identified by comparing and analyzing the sequences. For salmonid-derived products that were composed of a single species， the results indicated that species identification could be achieved by using COI as the target and 16S rRNA as the auxiliary target. Thus， our study confirmed DNA barcoding as an effective tool to identify the authenticity of salmonids， which could provide strong technical supports for supervision of salmonids market.