细点圆趾蟹含肉下脚料酶解物中抗氧化肽的分离与鉴定
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浙江省科技厅优先主题重点农业项目(2011C12041)


Isolation and Identification of Antioxidant Peptides Derived from Ovalipes punctatus Waste Containing Meat Scraps
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    摘要:

    目的:从细点圆趾蟹含肉下脚料的胰蛋白酶酶解产物中分离鉴定具有高ABTS+·清除率的抗氧化肽。方法:通过超滤系统、Sephadex G-15和半制备型RP-HPLC,从细点圆趾蟹含肉下脚料酶解产物中分离制备抗氧化肽,用超高效液相色谱-电喷雾飞行时间串联质谱(UPLC-ESI-TOF-MS/MS)对肽的结构进行表征,再通过合成肽对其结构与ABTS+·清除率进行验证。结果:获得2个ABTS+·清除能力较强的抗氧化肽,其氨基酸序列分别为Tyr-Glu-Gly(YEG)和Tyr-Glu(YE)。合成肽YEG和YE的分子质量分别为367.35 u和310.30 u,纯度分别为98.52%和98.04%,清除ABTS+·的IC50值分别为0.456 mg/mL和0.184 mg/mL,且YE的IC50值比L-还原型谷胱甘肽高30.30%。结论:细点圆趾蟹含肉下脚料的胰蛋白酶酶解产物含有抗氧化活性的肽类成分,为其抗氧化肽的开发提供理论依据。

    Abstract:

    Objective: Isolation and identification of antioxidant peptide with high ABTS+· scavenging capacity from tryptic hydrolysate of Ovalipes punctatus waste containing meat scraps. Method: Antioxidant peptides in the hydrolysates were purified using ultrafiltration membrane system, Sephadex G-15 column and semi-preparative reversed-phase high-performance liquid chromatography (semi-preparative RP-HPLC). Their peptide sequences were identified by ultra-performance liquid chromatography/electrospray ionization time-of-flight tandem mass spectrometry(UPLC-ESI-TOF/MS/MS). Their structure and ABTS+· scavenging capacity were further verified by the synthetic peptides. Results: Two peptides with high ABTS+· scavenging capacity were purified from hydrolysates, and their peptide sequences were Tyr-Glu-Gly (YEG) and Tyr-Glu(YE), respectively. The molecular weight of synthetic peptides was 367.35 u and 310.30 u, and their purity was 98.52% and 98.04%, respectively. The synthetic peptides YEG and YE have good ABTS+· scavenging capacity, with IC50 values of 0.456 mg/mL and 0.184 mg/mL, respectively. Furthermore, IC50 value of synthetic peptide YE was 30.30% higher than that of reduced L-glutathione. Conclusion: Antioxidant peptides were existed in the tryptic hydrolysate of Ovalipes punctatus waste containing meat scraps, which provides a theoretical basis for development of antioxidant peptides from Ovalipes punctatus by-products.

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余辉;陈小娥;曾茂茂;何志勇;张爽;方旭波;陈洁.细点圆趾蟹含肉下脚料酶解物中抗氧化肽的分离与鉴定[J].中国食品学报,2020,20(9):241-247

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  • 在线发布日期: 2020-10-13
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