大豆种皮多糖对模拟肠液流变特性和肠道菌群的影响
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国家自然科学基金项目(31901680)


Effects of Soy Hull Polysaccharide on the Rheological Properties of Simulated Intestinal Fluid and Intestinal Flora
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    摘要:

    采用微波辅助萃取剂提取大豆种皮多糖,探究大豆种皮多糖对模拟肠液流变特性的影响及其对肠道菌群的调节作用。以草酸为萃取剂,萃取得到大豆种皮多糖(MOSP);以柠檬酸为萃取剂,萃取得到大豆种皮多糖(MCSP),并检测其基本成分。将MOSP和MCSP添加到培养基中进行发酵,检测模拟肠液的OD值、pH值、流变特性等指标,并通过Illumina-Miseq高通量测序技术分析肠道菌群的动态变化。结果表明,MOSP得率为10.2%,MCSP得率为5.9%,MOSP的纯度、水分和脂肪含量较MCSP高;0~24 h内MOSP促进肠道菌群增殖与产酸的效果优于MCSP,MOSP发酵液24 h时表观黏度最高;高通量测序结果显示,MOSP和MCSP均可增加拟杆菌门和厚壁菌门的含量,MOSP比MCSP对多糖利用菌的促增殖效果更明显。2种大豆种皮多糖均在一定程度上改变体外模拟肠液的流变学特性,改善肠道菌群的多样性与结构特征,其中MOSP的作用效果更好。

    Abstract:

    Soy hull polysaccharide was extracted by microwave-assisted extractant method, and the effect of soy hull polysaccharide on the rheological properties of simulated intestinal fluid and its regulation on the intestinal flora were investigated. The basic components of soy hull polysaccharide (MOSP) extracted by oxalic acid and soy hull polysaccharide (MCSP) extracted by citric acid were detected. The OD value, pH value and rheological indicator of simulated intestinal fluid were detected after MOSP and MCSP were added to the medium for fermentation. The dynamic changes of intestinal flora were analyzed through Illumina-miseq high-throughput sequencing technology. The results showed that the yield of MOSP was 10.2% and that of MCSP was 5.9%. The purity, moisture and fat content of MOSP were higher than MCSP. The effects of MOSP on promoting intestinal flora proliferation and acid production within 0-24 h was better than that of MCSP. The apparent viscosity of MOSP was the highest at fermentation 24 h. The high-throughput sequencing results showed that both MOSP and MCSP could increase the content of bacteroidetes and firmicutes, and MOSP had a more obvious effect than MCSP. Both soy hull polysaccharides could change the rheological properties and improve the diversity and structural characteristics of the intestinal flora in vitro intestinal simulation system, and the MOSP could achieve better results.

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黄靖航;杨立娜;赵亚凡;林芊;韩琳;雒明朔;王胜男;宋虹;周大宇;刘 贺.大豆种皮多糖对模拟肠液流变特性和肠道菌群的影响[J].中国食品学报,2020,20(12):48-55

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  • 在线发布日期: 2021-01-12
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