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植物乳杆菌DL3对腐败希瓦氏菌的抑制作用
刘佳伊;吕欣然;李婧;白凤翎;董君;励建荣
作者单位
刘佳伊;吕欣然;李婧;白凤翎;董君;励建荣 渤海大学食品科学与工程学院辽宁省食品安全重点实验室生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心宁夏出入境检验检疫局检验检疫综合技术中心 
摘要:
研究传统东北酸菜中分离的植物乳杆菌DL3对腐败希瓦氏菌的拮抗作用。通过分析腐败希瓦氏菌的最小抑菌浓度(MIC)、生长曲线、细胞膜通透性、紫外吸收物质、蛋白质合成等指标,探究植物乳杆菌对革兰氏阴性菌的抑菌机制。结果表明:植物乳杆菌DL3上清液对腐败希瓦氏菌的MIC为8.0 mg/mL。在腐败希瓦氏菌培养6 h后加入植物乳杆菌DL3上清液,受试菌稳定期的细胞量仅为对照组的一半,细胞数量下降1.16 lg(CFU/mL);培养20 h,添加植物乳杆菌DL3上清液的腐败希瓦氏菌电导率为34.3 ms/cm,对照组为33.1 ms/cm,且试验组OD260nm值比对照组高出11.95%,表明胞内核酸物质泄出。SDS-PAGE结果表明:经植物乳杆菌处理的腐败希瓦氏菌中蛋白质条带数量减少,颜色变浅。扫描电镜显示腐败希瓦氏菌细胞壁被破坏,细胞质泄露。植物乳杆菌DL3通过影响受试菌细胞膜的通透性,破坏细胞的完整性,导致胞内核酸外泄,影响细菌蛋白质的合成,最终腐败希瓦氏菌菌体细胞破裂、死亡。
关键词:  植物乳杆菌  腐败希瓦氏菌  抑菌机制
DOI:
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基金项目:辽宁省教育厅高水平创新团队国(境)外培养项目〔辽教函(2018)225号〕
Antibacterial Effect of Lactobacillus plantarum DL3 against Shewanella putrefaciens
Abstract:
The antagonism effect of Lactobacillus plantarum DL3 isolated from traditional northeast suan-tsai against Shewanella putrefaciens was studied. Through the analysis of the minimum inhibitory concentration (MIC), growth curve, cell membrane permeability, ultraviolet absorption material, protein synthesis and other indicators of S. putrefaciens, the antibacterial effects of lactic acid bacteria(LAB) against Gram-negative bacteria were preliminarily investigated. The results showed that the MIC of strain DL3 cell free supernatant (CFS) against S. putrefaciens was 8.0 mg/mL. L. plantarum DL3 CFS was added after S. putrefaciens culturing for 6 h, the steady-state cell growth of the growth curve of test bacteria was only one-half of the control group, and the number of cells decreased by 1.16 lg (CFU/mL). The conductivity of S. putrefaciens with L. plantarum DL3 CFS was 34.3 ms/cm, and that of the control group was 33.1 ms/cm, and the OD260nm value of the experimental group was 11.95% higher than that of the control group, indicating that the intracellular nucleic acid substance released. SDS-PAGE confirmed that the number of protein bands in S. putrefaciens treated with Lactobacillus plantarum was small and the color was light. Scanning electron microscopy revealed that the cell wall of the S. putrefaciens cells was destroyed and the cytoplasm leaked. L. plantarum DL3 affected the permeability of the cell membrane of the test bacteria, destroyed the integrity of cells, caused leakage of intracellular nucleic acids, and affected the synthesis of bacterial proteins, led to leakage of proteins, caused cell death of S. putrefaciens.
Key words:  Lactobacillus plantarum  Shewanella putrefaciens  antibacterial mechanism

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