Objective： The effects of different molecular weight Auricularia auricular fermentation products on glucose lipid metabolism of HepG2 cells were evaluated by establishing HepG2 cell fat accumulation model and normal HepG2 cell model. The effects and differences of glucose lipid metabolism in different molecular weights of A. auricular were revealed. Methods： The supernatant of A. auricular fermentation was divided into 0-10， 10-50， 50-100， 100-300 ku and >300 ku by ultrafiltration centrifuge tube， and the fermentation products of A. auricular was obtained by constant temperature drying. The HepG2 cell fat accumulation model and the normal HepG2 cell model was established. And the effects of different molecular weights fermentation products of A. auricular on glucose lipid metabolism were evaluated by thiazolyl staining absorbance （MTT value）， oil red O staining， triglyceride （TG） value， total cholesterol （TC） value， lipoprotein lipase （LPL）， Hepatic lipase （HL） activity， glucose content， hepatic glycogen content and hexokinase （HK） activity. Results： The fermentation products of A. auricular can regulate the glucose lipid metabolism of HepG2 cells， reduce cell fat accumulation and glucose content， and increase the activity of fat metabolism enzyme and sugar metabolism enzyme. Among them， the effects of 100-300 ku and >300 ku A. auricular fermentation products were more obvious. Conclusion： The fermentation products of A. auricular can regulate glucose lipid metabolism in HepG2 cells， and the effects of 100-300 ku and >300 ku A. auricular fermentation products are more obvious.