黑木耳胞外多糖对小鼠肠道微生态及免疫调节的影响
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国家重点研发计划项目(2018YFD0400500,2017YFD0400300);全军重点科研项目(BX115 C007)


Effects of Exopolysaccharides from Auricularia Auricula-judae on the Intestine Microecology and Immunomodulatory in Mice
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    摘要:

    黑木耳作为一种传统的食用真菌,具有丰富的营养价值和多种活性功能,然而其生物活性成分对肠道微生物的调节作用还不清楚。本研究以黑木耳液体发酵产物为原料,采用水提醇沉法提取发酵液中的多糖,结合红外光谱、高效液相、16S rRNA测序技术、气相色谱和酶联免疫(ELISA)试验,分析黑木耳胞外多糖的组成及对小鼠肠道菌群多样性、肠道短链脂肪酸含量和血清细胞因子水平的影响。结果表明,黑木耳胞外多糖是以葡萄糖和木糖为主要单糖组分的杂合多糖,能够显著提高小鼠肠道内拟杆菌属和罗氏菌属的水平(P<0.05);增加肠道内丙酸和丁酸的含量,其中丙酸含量为对照组的3.3倍(P<0.01),丁酸含量为对照组的1.4倍(P<0.01);提升血清中细胞因子白介素-10(IL-10)的含量(P<0.05),而对肿瘤坏死因子(TNF-α)和白介素-6(IL-6)的水平无显著影响(P>0.05)。本研究表明,黑木耳胞外多糖具有促进肠道内有益菌群增殖,增加短链脂肪酸含量及上调血清抗炎因子水平的作用。

    Abstract:

    Auricularia auricula-judae has a variety of medicinal value and healthy function, however, its roles in the regulation of intestinal microbes and the products of microbial metabolism are still unclear. In this study, the exopolysaccharides were extracted from the fermentation culture of Auricularia auricula-judae using alcohol precipitation method. Fourier transform infrared spectrometry (FT-IR), high-performance liquid chromatography (HPLC), 16s rRNA gene sequencing analysis, gas chromatography and ELISA were used to detect the composition of exopolysaccharides and the changes of intestinal flora, short-chain fatty acid and cytokines in the mice treated with Auricularia auricula-judae exopolysaccharides. The results show that the exopolysaccharides from Auricularia auricula-judae fermentation are mainly composed of glucose and xylose. Auricularia auricula-judae exopolysaccharides significantly increase the levels of Bacteroides and Roseburia in intestine (P<0.05), and enhance the abundance of propionic acid (3.3 times vs the control group, P<0.01) and butyric acid (1.4 times vs the control group, P<0.05) in feces. Although the levels of cytokine TNF-α and IL-6 in the mice are not significantly affected by the Auricularia auricula-judae exopolysaccharides treatment (P>0.05), it promote the level of IL-10 in the serum (1.3 times vs the control group, P<0.01). In summary, our study indicates that Auricularia auricula-judae exopolysaccharides promote the abundance of probiotics in the mice intestine, and regulate the metabolism of short-chain fatty acids and the levels of cytokine.

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郝敏;李殿龙;徐俊亭;蔺洁茹;王旭;施治臣;郝利民;刘振兴.黑木耳胞外多糖对小鼠肠道微生态及免疫调节的影响[J].中国食品学报,2021,21(3):63-70

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  • 在线发布日期: 2021-04-19
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