枯草芽孢杆菌中胞壁肽的分离纯化及其对芽孢的影响
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国家自然科学基金重点项目(31530058)


The Isolation and Purification of Muropeptides from the Bacillus subtilis and Its Effect on Spore Germination
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    摘要:

    胞壁肽是来源于细菌细胞壁中肽聚糖酶解后产物,具有促进芽孢萌发的生理功能。本文利用枯草芽孢杆菌为对象,通过酶解制备枯草芽孢杆菌中胞壁肽。利用反式高效液相色谱仪对制备的胞壁肽进行分离,并利用液相-质谱联用、傅里叶变换红外光谱、核磁共振方法对胞壁肽进行结构鉴定。还研究了胞壁肽对芽孢萌发的影响。结果表明:分离纯化后得到的胞壁肽最小结构单元为二糖三肽,胞壁肽可促进90%芽孢萌发。

    Abstract:

    The muropeptides was the fractions of peptidoglycan from cell wall after digested by mutanolysin. It could induce the spore germination. In this paper, the muropeptides were prepared from the cell wall of Bacillus. subtilis 168 by the method of enzymatic hydrolysis. Then, the high-performance liquid was used to separate and purified the muropeptides. In addition, the structure of muropeptides was identified by liquid chromatography-mass spectrometry, Fourier transform infrared spectroscopy and nuclear magnetic resonance, and the effect of muropeptides on spore germination was further studied. The results showed that the smallest unit of muropeptides obtained after separation and purification was disaccharide tripeptide, and it could induce about 90% spore to germinate.

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梁栋;陈芳;张良;胡小松.枯草芽孢杆菌中胞壁肽的分离纯化及其对芽孢的影响[J].中国食品学报,2021,21(3):369-374

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  • 在线发布日期: 2021-04-19
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