库车小白杏仁内生细菌的筛选及其功能特性初探
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国家重点研发计划项目(2018YFC1603400);新疆维吾尔自治区自然科学基金项目(2018D 01A15)


Isolation of Endophytic Bacteria from Small White Almond of Kuqa County and Preliminary Studies on Their Functional Characteristics
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    摘要:

    为探究库车小白杏仁内生细菌群落多样性,挖掘其中潜在的功能微生物资源,利用Biolog-ECO微平板技术结合传统分离培养法,对库车小白杏仁内生细菌群落碳源的利用类型进行分析,并对其内生细菌进行筛选与鉴定。同时确定所得菌株的产酶特性和拮抗作用。结果显示,库车小白杏仁内生细菌群落主要利用的碳源类型为碳水化合物类和氨基酸类,约占总体的77%。其中,D-半乳糖醛酸、D-甘露醇和N-乙酰-D-葡萄糖胺为主要的碳水化合物类型;L-天门冬酰胺为主要的氨基酸类型。试验获得的47株内生细菌,分别归属于变形杆菌门、厚壁菌门、放线菌门、拟杆菌门4个门的13个属,发现潜在新种1个。多数菌株可产蛋白酶、脂肪酶、淀粉酶、果胶酶,以及具有良好的拮抗作用。上述研究表明,库车小白杏仁内生细菌群落存在丰富的生物多样性,为进一步开发和利用相关菌株的功能特性提供试验依据。

    Abstract:

    In order to investigate the diversity of endophytic bacteria in Kuqa almond and collect potential microbial resources. In this paper, Biolog-ECO microplate method and culture method was used to isolate and screen endophytic bacteria from Kuqa almond, and characteristics of enzyme production and antagonism of the strains were analyzed. The results showed that main utilization of carbon sources were carbohydrate and amino acid, accounting for about 77% of the total. D-galacturonic acid, D-Mannitol, N-acetyl-D-glucosamine and L-asparagine were major compounds utilized by endophytic bacteria. Meanwhile, 47 isolates belonged to 13 genera of Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes were obtained, and one potential new species was observed. Additionally, a number of bacteria producing protease, lipase, amylase, pectinase and antagonism was found. In a conclusion, diversity of endophytic bacteria in Kuqa almond was rich, which provided a research basis for utilization of related functional strains.

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刘晓静;梁幸豪;朱静;楚敏;顾美英;唐琦勇;张志东.库车小白杏仁内生细菌的筛选及其功能特性初探[J].中国食品学报,2021,21(6):280-286

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  • 在线发布日期: 2021-07-16
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