细胞水平染料木素协同维生素D3的抗骨质疏松作用
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国家自然科学基金面上项目(21878041)


Analysis of Interaction Between Freshwater Clam Soup and Rat Macrophages by Capillary Electrophoresis
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    摘要:

    维生素D3(Vitamin D3,VD3)和染料木素(Genistein,Gen)二者均具有抗骨质疏松作用,然而,二者协同作用机制及维生素D3的抗骨质疏松作用使用剂量有待阐明。本研究报告了维生素D3和染料木素对人成骨肉瘤细胞MG-63、小鼠巨噬细胞RAW264.7诱导的破骨细胞增殖与分化的影响机制。维生素D3联合染料木素可显著促进成骨细胞的增殖分化,促进骨保护素(OPG)的表达,抑制核因子κB(NF-κB)受体激活剂(RANKL)的表达,提高OPG和RANKL mRNA表达比值。此外,维生素D3在6.25~50.00 μmol/L剂量范围,可促进破骨细胞的增殖和成熟,而联合染料木素后表现为显著抑制作用。维生素D3可促进破骨细胞中组织蛋白酶K的表达,联合染料木素处理细胞,显著抑制酒石酸抗性磷酸酶(TRAP)和组织蛋白酶K的表达。试验结果表明,维生素D3联合染料木素,可显著促进成骨细胞的增殖和分化。维生素D3表现为浓度依赖的增强破骨细胞增殖和成熟作用,而联合染料木素可显著抑制破骨细胞增殖和成熟,间接起到抗骨质疏松作用。

    Abstract:

    A new method of rapid monitoring the interaction between food components and macrophages was developed based on capillary zone electrophoresis. Previously, a method was established for rapid separation and quantification of self-assembled nanoparticles from freshwater clam (Corbicula fluminea Muller) soup by capillary zone electrophoresis. This study used the same technique to separate and analyze the rat peritoneal macrophages and multiple-scale components of freshwater clam soup and the direct interaction between the cells and the soup, during and after the co-incubation. The profile of soup components and the electrophoretic behavior and morphology of macrophages were monitored. The results indicate that macrophages were separated into fractions with distinguishable migration time, which was elongated by the elevated cell numbers. Macrophages engulfed the soluble components and nanoparticles of clam soup. The average number of nanoparticles engulfment per cell was 8.7×102 particles at 24 h and 2.13×103 particles at 48 h. The clam soup components stimulated the secretion of macrophages, while the nanoparticles decreased the cellular surface charge, accelerated the electrophoretic mobility and promoted the cell differentiation. In summary, capillary zone electrophoresis is capable of rapid monitoring the interaction between the multiple-scale food components and macrophages, quantifying the subsequent changes occurred in the components, particles numbers and their surface properties. It demonstrated a promising new approach for rapid analysis of the direct food-human interaction.

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赵佳;姜波;郭建丽;张丹洋;孙文辉;包永明.细胞水平染料木素协同维生素D3的抗骨质疏松作用[J].中国食品学报,2021,21(9):19-29

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  • 在线发布日期: 2021-10-19
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