离子色谱法测定母乳和牛乳中的N-乙酰神经氨酸
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Determination of N-Acetylneuraminic Acids in Human and Cow Milk by Ion Chromatography
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    摘要:

    目的:母乳及动物乳汁中含有丰富的N-乙酰神经氨酸。本研究拟建立方法用于测定不同乳汁中N-乙酰神经氨酸。方法:本文采用高效阴离子交换色谱偶联脉冲安培检测器法(HPAEC-PAD)建立并验证测定母乳及牛乳中总N-乙酰神经氨酸的方法。结果:方法前处理简单,在较宽的分析范围(20.7~4 000 mg/L)内线性良好,相关系数大于0.999,母乳样品中的检出及定量限分别为4.3 mg/L和20.7 mg/L,准确性和精密度良好,加标回收率在86.1%~99.3%之间,平均回收率92.7%,6次重复测量RSD低于1.9%。测定不同泌乳阶段的母乳及市售牛乳中N-乙酰神经氨酸的结果显示,产后1年内母乳中总N-乙酰神经氨酸含量在187.1~1 334.7 mg/L,其随泌乳期的延长呈下降趋势。其中,游离态N-乙酰神经氨酸的比例在1.0%~2.5%。5种市售牛乳中总N-乙酰神经氨酸的含量范围为177.6~228.9 mg/L,其中游离态N-乙酰神经氨酸的比例在8.5%~11.2%。结论:该方法可用于快速准确地测定母乳及牛乳中总N-乙酰神经氨酸的含量。

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    Objective: N-acetylneuraminic acid is widely present in human breast milk and animal milk. This study intends to establish a method for the determination of N-acetylneuraminic acid in different milks. Methods: The high-performance anion exchange chromatography coupled with pulsed amperometric detector method (HPAEC-PAD) was used to establish and verify a method for determining the content of total N-acetylneuraminic acid in breast milk and cow milk. Results: The method has simple pretreatment, excellent linearity in a wide linear range (20.7-4 000 mg/L), with a correlation coefficient greater than 0.999, and the limits of detection and quantification are 4.3 mg/L and 20.7 mg/L, respectively. The linear range and limit of quantification of the method were satisfied the need to determinate the content of N-acetylneuraminic acid in breast milk over 1 years and commercially available cow milk. The method is applicable to the determination of N-acetylneuraminic acid in different milks. The accuracy and precision of the method are good, and the recovery of spike addition is between 86.1%-99.3%. The RSD of six repeated measurements is less than 1.9%. The content of total N-acetylneuraminic acid in breast milk within a year after delivery is 187.1-1334.7 mg/L, and the content shows a downward trend along lactation period. The proportion of free N-acetylneuraminic acid in breast milk is 1.0%-2.5%. The content of N-acetylneuraminic acid in five kinds of commercially available cow's milk is 177.6-228.9 mg/L of which free N-acetylneuraminic acid accounted for 8.5%-11.2%. Conclusion: This method is suitable for rapid and accurate determination of total N-acetylneuraminic acid in breast milk and cow milk.

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刘爽;陈磊;Philip Haselberger;田芳;蔡小堃;毛颖异;王津;赵艳荣;王硕.离子色谱法测定母乳和牛乳中的N-乙酰神经氨酸[J].中国食品学报,2021,21(9):250-256

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  • 在线发布日期: 2021-10-19
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