枯草芽孢杆菌细菌素A32的抑菌机理研究
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山西省重点研发计划项目(201803D221009-5)


Studies on the Inhibition Mechanism of Bacteriocin A32 Producing by Bacillus subtilis
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    摘要:

    将酸沉淀法提取的枯草芽孢杆菌细菌素A32作用于大肠杆菌和金黄色葡萄球菌,利用牛津杯法、分光光度法、扫描电镜技术和电导率法、SDS-PAGE电泳法探讨细菌素A32的抑菌活性和抑菌机理。结果显示,细菌素A32对大肠杆菌和金黄色葡萄球菌均具有抑菌性,最小抑菌质量浓度为1.25 mg/mL;对指示菌的生长曲线、细胞形态结构、细胞膜渗透性、细胞膜通透性、细胞膜和细胞壁完整性、细胞中蛋白质的合成均有影响;且随着细菌素A32质量浓度的增大,对指示菌的影响也逐渐增大。以上现象表明,细菌素A32的抑菌性主要是通过破坏革兰氏阴、阳性菌壁膜的通透性和完整性,使内容物外漏,进而影响菌体代谢和生长。孔道形成理论是细菌素A32对革兰氏阴、阳性菌的作用机理。研究结果为细菌素A32作为绿色生物防腐剂的开发应用奠定了理论基础。

    Abstract:

    Bacteriocin A32 extracted by acid precipitation was used on Escherichia coli and Staphylococcus aureus. The antibacterial activity and antibacterial mechanism of bacteriocin A32 were investigated by Oxford Cup method, spectrophotometry, scanning electron microscope, electrical conductivity and SDS-PAGE method. The results showed that bacteriocin A32 had antibacterial properties against E. coli and S. aureus, and the minimum inhibitory concentration was 1.25 mg/mL. It had effects on the production curve of the indicator bacteria, cell morphology, cell membrane permeability, cell membrane permeability, cell membrane integrity, cell wall, and protein synthesis. And with the increase of the concentration of bacteriocin A32, the effect gradually increased. The results showed that the antibacterial properties of bacteriocin A32 were due to the damage to the permeability and integrity of the wall membrane of Gram-positive and Gram-negative bacteria, resulting in leakage of the contents, which in turn affected the metabolism and growth of the bacteria. The above research can lay a theoretical foundation for the development and application of bacteriocin A32 as green biological preservative.

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高娟娟,贾丽艳,畅盼盼,田宇敏,甄晓君,王恬恬.枯草芽孢杆菌细菌素A32的抑菌机理研究[J].中国食品学报,2021,21(10):56-64

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  • 在线发布日期: 2021-11-22
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