乳品中耐药微生物污染及blaTEM基因溯源
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国家重点研发计划项目(2018YFC1604303-04,2018YFC1604301-03); 北京市科技专项(Z191100001719006)


Contamination of Drug-resistant Microorganisms and Traceability of blaTEM Gene in Dairy Products
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    摘要:

    为了解乳品中耐药微生物及耐药基因主要污染源,以乳品及对应牧场环境样品中耐药微生物为研究对象,采用三磷酸腺苷(ATP)荧光检测及聚合酶链式反应(PCR)方法分析挤奶环节污染分布情况,构建分子发育树追溯blaTEM耐药基因潜在污染源。结果表明,挤奶环节中部分与外界接触较多的管路接口污染风险较高。不同牧场间微生物耐药性差别较大,其中氨苄西林抗性菌株检出率最高为61.5%,环境及乳样中β-内酰胺类抗生素blaTEM耐药基因检出率高达95.3%。挤奶是blaTEM基因潜在污染环节,饮用水可能是主要污染源。结论:牧场是乳品中耐药微生物及blaTEM耐药基因潜在污染环节,blaTEM耐药基因存在随生产链迁移的风险。

    Abstract:

    At present, systematic analysis of the main pollution sources of blaTEM resistance genes in dairy products in Beijing had not been reported. In order to understand the main pollution sources of drug-resistant microorganisms and drug-resistant genes in dairy products. It had been taken as the research object that drug-resistant microorganisms in dairy products and corresponding farm environmental samples, Adenosine triphosphate(ATP) fluorescence detection and Polymerase Chain Reaction(PCR) methods were used to analyzed the distribution of milking contamination and build a molecular development tree to traced the potential pollution sources of blaTEM drug resistance genes. The results showed that the contamination risk was relatively high of some pipeline interfaces with more contact with the outside world in the milking process, and there were great differences in microbial drug resistance among different pastures, in which the detection rate of ampicillin resistant strains at 61.5%. The detection rate was as high as 95.3% of β-lactam antibiotic blaTEM resistance gene in environment and milk samples, and milking was the blaTEM genes potential link of pollution, and drinking water might be the main source of pollution. In summary, the pasture is a potential contamination link of drug-resistant microorganisms and blaTEM resistance genes in dairy products. There is a risk that blaTEM resistance genes will migrate along the production chain.

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周 浩,栾林众,刘继超,赵军英,尹春媚,陈历俊.乳品中耐药微生物污染及blaTEM基因溯源[J].中国食品学报,2021,21(10):255-261

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  • 在线发布日期: 2021-11-22
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