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大蒜油自微乳的抑菌作用
肖小年;盛丹梅;周洁;易孜成;朱碧泉;张俊;易醒
作者单位
肖小年;盛丹梅;周洁;易孜成;朱碧泉;张俊;易醒 南昌大学中德联合研究院南昌大学中德食品工程中心 
摘要:
本文研究了大蒜油自微乳(GO-SMEDDS)对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、铜绿假单胞菌、白色念珠菌、酿酒酵母的抑制作用。通过测定抑菌圈直径,确定最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。以大肠杆菌和金黄色葡萄球菌为试验菌株,通过形貌观察,以及电导率、碱性磷酸酶(AKP)、谷丙转氨酶(ALT)和谷草转氨酶(AST)活性的测定,综合评价大蒜油自微乳的抑菌效果。结果表明:大蒜油自微乳的抑菌作用大小依次为:真菌、革兰氏阳性菌、革兰氏阴性菌,对大肠杆菌和金黄色葡萄球菌的最低抑菌质量浓度分别为1.875 mg/mL和0.469 mg/mL,最低杀菌质量浓度分别为3.750 mg/mL和0.938 mg/mL。大蒜油自微乳的抑菌机理是使大肠杆菌和金黄色葡萄球菌细胞壁和细胞膜受损,引起菌体细胞内碱性磷酸酶、谷丙转氨酶和谷草转氨酶泄露以及离子渗出,从而导致菌体的死亡。
关键词:  大蒜油自微乳  大肠杆菌  金黄色葡萄球菌  抑菌机理
DOI:
分类号:
基金项目:国家自然科学基金项目(31760459,31960455)
Inhibitory Effect of Garlic Oil Self-Microemulsion on Bacteria
Abstract:
The inhibitory effects of garlic oil self-microemulsion(GO-SMEDDS) on Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Candida albicans and Saccharomyces cerevisiae were investigated in this paper. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of GO-SMEDDS were determined by measuring the diameter of the inhibitory circle. Escherichia coli and Staphylococcus aureus were applied to use as test strains, and then the inhibitory effect of GO-SMEDDS on bacteria was evaluated by the combination of morphology, electrical conductivity and the activity of Alkaline phosphatase(AKP), Alanine Aminotransferase (ALT) and Aspartic Transaminase(AST). The results indicated that the potential ability of the bacteriostatic effects of GO-SMEDDS were in high to low order as follows: fungi, gram-positive bacteria and gram-negative bacteria. The MIC of GO-SMEDDS against Escherichia coli and Staphylococcus aureus were 1.875 mg/mL and 0.469 mg/mL, respectively, and the MBC were 3.750 mg/mL and 0.938 mg/mL, respectively. The antibacterial mechanism of GO-SMEDDS on Escherichia coli and Staphylococcus aureus is that the cell walls and membranes are damaged, which causes the leakage of AKP, ALT, AST in the bacteria cells, thus leading to the death of the bacteria.
Key words:  garlic oil self-microemulsion  Escherichia coli  Staphylococcus aureus  antibacterial mechanism

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