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真空低温喷雾干燥制备乳双歧杆菌Probio-M8微胶囊
郭帅;王昊乾;徐鹏飞;许家齐;郑新飞;张和平1,2
1.内蒙古农业大学;2.乳品生物技术与工程教育部重点实验室
摘要:
目的:采用真空低温喷雾干燥制备乳双歧杆菌Probio-M8微胶囊。方法:通过单因素试验、正交试验及Box-Behnken响应面试验确定最佳工艺条件,采用气相色谱法检测干燥前、后菌体细胞膜脂肪酸组分变化,并用流式细胞仪观测干燥前、后菌体细胞膜完整性的变化;采用扫描电镜观察微胶囊粉的形态结构以及使用差示扫描量热仪测定微胶囊粉的玻璃化转变温度。结果:最佳的喷雾条件:进风温度80 ℃、进料速度9 mL/min、喷头直径1.5 mm。最佳复配保护剂配方:脱脂乳添加量13%、麦芽糊精10%、谷氨酸钠7%。在最佳工艺条件下生产的Probio-M8微胶囊存活率为78.34%。其结构完整,常温下处于玻璃态,菌体细胞膜的完整性也显著提高,干燥后菌体细胞膜脂肪酸的UFA/SFA比值提高了0.44,稳定了细胞膜的流动性。结论:通过优化工艺条件,显著地维持了菌体的生物活性,为生产乳双歧杆菌Probio-M8微胶囊提供了一种低能耗、高活性的技术方案。
关键词:  真空低温喷雾干燥  乳双歧杆菌Probio-M8  微胶囊
DOI:
分类号:
基金项目:内蒙古自治区科技重大专项(zdzx2018018);国家现代农业产业技术体系建设项目
Preparation of Bifidobacterium lactis Probio-M8 Microcapsule by Vacuum Low Temperature Spray Drying
Abstract:
Objective: The preparation of Bifidobacterium lactobacillus Probio-M8 microcapsules by vacuum low temperature spray drying was studied in this paper. Methods: The optimum process conditions were determined by single factor, orthogonal experiments and Box-Behnken response surface tests. The changes in the fatty acid composition of the bacterial cell membrane before and after drying was analyzed by the gas chromatographic analysis. The impact of vacuum low temperature spray drying on the membrane integrity was investigated by the flow cytometry. The microcapsule morphological structure was observed through scanning electron microscopy. And the glass translation temperature of this microcapsule was determined by the differential scanning calorimetry. Results: It was found that the optimum spray processing parameters were inlet air temperature of 80 ℃, material flow of 9 mL/min and nozzle diameter of 1.5 mm; the best formulation of protective agent was 13% skim milk, 10% maltodextrin and 7% monosodium glutamate. Under the optimum conditions of spray drying, the survival rate of Probio-M8 was 78.34%, the microcapsule had complete structure and its state was glassy at room temperature, the integrity of cell membrane was also significantly improved. After drying, the UFA/SFA ratio of fatty acids in cell membrane increased by 0.44, maintaining the fluidity of the cell membrane. Conclusion: By optimizing the process conditions, this experiment significantly maintained the biological activity of the bacteria, and provided a technical scheme for the Probio-M8 microcapsules with low energy consumption and high activity.
Key words:  vacuum low temperature spray drying  Bifidobacterium lactis Probio-M8  microcapsule

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