Objective： Complex plant-source extracts of large yellow croaker （Pseudosciaena crocea） during ice storage were optimized by response surface methodology. Methods： TVB-N value of large yellow croaker （Pseudosciaena crocea） with different plant-source extract treatments during ice storage can be measured and the optimum concentration of Ginkgo biloba leaves extract （GBLE）， Bamboo vinegar （BV） and tea polyphenol （TP） on large yellow croaker （Pseudosciaena crocea） during ice storage were measured by single-factor experiment respectively， the optimal ratio of complex plant-source extracts were analyzed by response surface methodology. Results： The optimal mass fraction of GBLE， BV and TP were 0.5%， 0.5% and 1.5% respectively. The interaction of BV and TP solution had significant effect on TVB-N value （P<0.05）， the optimal ratio of three kinds of plant-source bio-preservatives was 1.38% of GBLE， 0.92% of BV and 0.85% of TP. Conclusion： The predicted value of TVB-N was 47.17 mg N/100 g and the measured value was 50.80 mg N/100 g. Therefore， the response surface regression model was reliable.