海南地区健康青年肠道中乳酸菌和双歧杆菌多样性研究
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国家重点研发计划项目(2017YFD0400204)


Studies on Diversity of Lactic Acid Bacteria and Bifidobacterium in the Intestine of Healthy Youth in Hainan Province
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    摘要:

    目的:在种的水平揭示海南地区健康青年肠道中乳酸菌和双歧杆菌的丰度和生物多样性,探讨性别、年龄和BMI对上述两者可能的影响。方法:以海南地区27名健康志愿者为研究对象,将乳酸菌特异性引物和双歧杆菌特异性引物扩增与PacBio SMRT测序技术相结合,采用相关生物信息学与统计学方法分析。结果:志愿者肠道中乳酸菌主要由链球菌属、乳杆菌属、魏斯氏菌属、瘤胃球菌属等组成。按照乳酸菌在种水平的组成,所有人群可以分为两个肠型,两个肠型人群唾液链球菌的比例存在显著差异(P<0.05)。此外,男性志愿者肠道中乳酸菌丰度和多样性显著高于女性。依据肠道双歧杆菌多样性特征,可将志愿者分为以链状双歧杆菌、长双歧杆菌、粪双歧杆菌为优势菌的3个类群,性别和BMI均是对双歧杆菌组成产生影响的因素。结论:青年人群肠道中具有丰富的乳酸菌和双歧杆菌,其构成与个人饮食习惯、性别和BMI有一定相关性,肠道中优势乳酸菌和双歧杆菌对人体健康发挥着重要作用。

    Abstract:

    In this study, we investigated the abundance and diversity of lactic acid bacteria(LAB) and Bifidobacterium in 27 healthy young people from Hainan province using laboratory-designed LAB primers and Bifidobacterium-specific primers; this work also aimed to discuss the influence of sex, age, and body mass index (BMI) on the quantities of these two bacterial groups. The fecal LAB in the volunteers were mainly composed of Streptococcus, Lactobacillus, Weissella and Ruminococcus. According to the composition of LAB at species level, all volunteers could be divided into two enterotyping. The proportion of Streptococcus salivarius in the two enterotyping had significant difference(P<0.05). The abundance and diversity of LAB in the gut of male volunteers were significantly higher than that in women. In addition, volunteers’ could be divided into three different groups according to the diversity characteristics of bifidobacteria. The representative bifidobacterial strains are B. catenulatum, B. longum and B. stercoris, respectively. Both gender and BMI are factors influencing the composition of bifidobacterial. This study has established accurate methods in determining LAB and bifidobacteria in human intestinal samples, as well as preliminary described the LAB and bifidobacteria subpopulations in subjects of Hainan province. Results obtained in this paper serve as good reference for other studies.

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温永平;沈玲玲;孙志宏;江正强.海南地区健康青年肠道中乳酸菌和双歧杆菌多样性研究[J].中国食品学报,2019,19(11):14-24

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  • 在线发布日期: 2019-12-02
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