朝鲜蓟茎叶副产物青贮过程中多酚及其功能活性的研究
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公益性行业(农业)科研专项(201503142)


Studies on Polyphenol and Its Function Activity of By-production of Artichoke’s Stem and Leaves during the Ensile
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    摘要:

    朝鲜蓟是一种具有保肝、抗癌、抗氧化、抗菌等活性的蔬菜,其茎叶等副产物也具有一定的生理活性,然而其含水量大、易腐,难以实现精深加工。本研究采用低能耗的青贮方式贮藏朝鲜蓟茎叶副产物,探究青贮期间其多酚类功能成分及抗氧化、抑制α-葡萄糖苷酶等生理活性的变化。采用HPLC-QQQ-MS检测单体酚的变化,用福林酚法测定其青贮期间总酚含量的差异,以抗氧化能力及抑制α-葡萄糖苷酶作为功能活性指标,对朝鲜蓟副产物青贮期间多酚的功能活性进行分析。结果表明:青贮能较好地保存朝藓蓟茎叶副产物中的咖啡酰奎宁酸、芦丁和山奈酚-3-O-葡萄糖苷,其总酚保留率达96.8%。对朝鲜蓟多酚活性的研究表明,青贮较好地维持了朝鲜蓟多酚粗提物的抗氧化活性,朝鲜蓟多酚对α-葡萄糖苷酶的抑制作用显著,较好地保留了朝鲜蓟茎叶副产物中的多酚成分,且不破坏其抗氧化及抑制α-葡萄糖苷酶的生理活性,是一种低能耗、高效的朝鲜蓟茎叶副产物保存方式,具有推广潜力。

    Abstract:

    Artichoke is a kind of vegetable with liver protection, anticancer, antioxidation and antibacterial activity, its by-products (leaves and stalks) also rich in beneficial physiological activity, but it is difficult to make intensive use of the by-products as they are too wet to be decay. In this study, silage, a low energy method, was use for storaging artichoke stems and leaves, meanwhile, the content of polyphenol and its antioxidant properties and the ability of inhibition of α-glucosidase was detected in ensile. In short, the changes of mono-phenols was detected by HPLC-QQQ-MS, and the alteration of total phenolic content in ensilage was determined by with Folin-Ciocalteu method. Meanwhile, antioxidant properties and the ability of inhibition of α-glucosidase was regarded as evaluating indicator of function activity. As result, there are still 96.8% of total polyphenol in the by-product after ensiling. Moreover, antioxidant properties is same to before, and the ability of inhibition of α-glucosidase is still significant. Consequently, ensilage can be a developed method to preserve the polyphenol of artichoke by-product and function activity as before, so it is a low energy and efficient method to preserve the by-products of artichoke stem and leaf that has potential for popularization.

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戴倩倩;樊雨梅;张晓旭;倪元颖;马丽艳;.朝鲜蓟茎叶副产物青贮过程中多酚及其功能活性的研究[J].中国食品学报,2020,20(2):263-270

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  • 在线发布日期: 2020-03-07
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