In the post-harvest storage process， blueberries often show softening and decay， which seriously affected the quality of blueberries. In this study， the ‘Lanfeng’ blueberry fruit in the process of postharvest storage at room temperature was tested. Through isolation and identification of surface pathogenic fungi in blueberry during storage at room temperature， clear the main pathogenic bacteria species of blueberry rot during storage at room temperature. The traditional fungi method was used to culture and purify pathogenic fungi， after morphological observation and analysis， Preliminary identification of blueberry surface pathogenic fungi species， and to carry out the return test to further determine the surface pathogenicfungi species. The ITS region of the isolated blueberry pathogenic fungi was amplified by PCR using the specific primers of ITS. The results showed that the surface pathogenic fungi of ‘Lanfeng’ blueberries at room temperature were penicillium， Penicillium chrysogenum and Alternaria alternata. Finally determine the optimum growth temperature of the strain. The optimum growth temperatures of Penicillium chrysogenum， Penicillium chrysogenum and Alternaria alternata were 28 ℃， 26-28 ℃ and 26 ℃， respectively. The study provided the theoretical basis for the prevention and control of disease in the preservation of blueberry fruit in Liaoning province.