基于BALB/C小鼠模型评价牛乳α_(s1)-酪蛋白的致敏特性
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国家自然科学基金项目(31872886);国家重点研发计划项目子课题(2018YFC1604205);国家重点研发计划项目(2019YFC1605000)


Allergic Mechanism of αs1-casein in Cow Milk Based on BALB/C Mice Model
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    摘要:

    目的:评价过敏原的过敏症机理。方法:首先通过口腔灌胃的致敏方式建立αs1-酪蛋白致敏的BALB/C小鼠模型,通过测定小鼠特异性IgE抗体水平进行模型鉴定。评价αs1-酪蛋白对小鼠肥大细胞蛋白酶、组胺、细胞因子水平的影响,并进行临床和病理切片观察。结果:第42天用αs1-酪蛋白大剂量灌胃小鼠后,各组小鼠特异性IgE抗体水平显著升高,都产生不同程度的过敏症状,且中、高剂量组小鼠过敏反应强于低剂量组;以阴性小鼠为对照,致敏小鼠的肥大细胞蛋白酶、组胺、IL-4、IL-5、IL-10和IFN-γ细胞因子含量均显著升高,且随着致敏剂量的增加而增加;组织病理切片结果显示:αs1-酪蛋白中剂量和高剂量组小鼠产生了明显的炎症反应,局部肺泡隔塌陷或增厚,脾脏和胸腺中有淋巴结病灶产生,且小肠黏膜间质有炎细胞浸润。结论:基于BALB/C小鼠模型进一步揭示了αs1-酪蛋白的致敏机理。

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    Objective: In order to evaluate the mechanism of allergen hypersensitivity. Methods: A mouse model of BALB/C sensitized by αs1-casein was established by oral gavage, and the levels of specific IgE antibodies were used to identify this animal model. The contents of mast cell proteinase, histamine and cytokines in mice were determined, and the clinical and pathological sections were observed. Results: The levels of specific IgE in each group mice increased significantly after the last large dose of αs1-casein was administered to mice at 42th day, and had different degrees of allergic symptoms. Moreover, the allergic reaction in medium and high dose group was stronger than that in low dose group. The levels of mast cell proteinase, histamine, IL-4, IL-5, IL-10 and IFN- gamma cytokines in sensitized mice were significantly higher than those in negative group, and increasing with the accumulation of sensitizing doses. The histopathological section showed that in the medium and high dose groups immunized with αs1-casein, the mice showed obvious inflammatory response, the local alveolar septum collapsed or thickened, and lymphatic foci were produced in the spleen and thymus, and the inflammatory cells infiltrated in small intestinal mucosa mesenchyme. Conclusions: Based on the BALB/C mouse model, the allergic mechanism of alpha αs1-casein was further revealed.

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丛艳君;刘家琦;于晓凤;吕晓哲;李晔;.基于BALB/C小鼠模型评价牛乳α_(s1)-酪蛋白的致敏特性[J].中国食品学报,2020,20(3):21-30

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  • 在线发布日期: 2020-04-14
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