Quorum sensing haII/haIR gene was used for this study and 16S rRNA was used as the reference gene， the expression of quorum sensing haII/haIR gene in different environments was determined by real-time fluorescence quantitative PCR to explore the mechanism of different environmental factors on quorum sensing stability. The results showed that the haII gene expression under different carbon source culture conditions was xylose> fructose> glucose> maltose> lactose> sucrose， and the expression of haIR gene was xylose > fructose > sucrose > glucose > lactose > maltose. The expression of haII/haIR gene were inhibited as sodium chloride concentration increased， and haII/haIR genes could be expressed under acid conditions. At the same time， genes expression had a significant up-regulation at 15 ℃， however beyond the optimum temperature， the expression decreased. Our results had an important implication for enriching the quorum sensing theory and expanding application of quorum sensing theory in the preservation of aquatic production.