Objective： To develop a rapid and simple method for the quantification of 28 kinds of synthetic colorant in meat product by ultra performance liquid chromatography quadrupole electrostatic field obitrap high resolution mass spectrometry. Methods： The samples were extracted by acetonitrile and ethyl acetate and cleaned up. The column was used as chromatography column to separate the synthetic colorant with a gradient elution of the mobile phase. The 28 kinds of synthetic colorant were analyzed in positive and negative mode. The retention time，accurate mass of parent ion and relative isotope abundance were used， while accurate mass of fragment ions was used for confirmation. Results： The linearities were good （R2 > 0.99）. The limits of detection （LODs） were between 2.5 and 500 μg/kg. The recoveries were in the range of 73.5%-109.2% and the relative standard deviations （RSDs） were 4.9%-10.0%. Conclusion： The method is simple， rapid and reliable，which is suitable for the rapid qualitative screening and quantitative analysis of 28 kinds of synthetic colorant in meat product.