酶法改性对米糠蛋白凝胶硬度及功能性质的影响
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“十三五”国家重点研发计划(2018YFD0401101)


Effects of Enzymatic Modification on Gel Hardness and Functional Properties of Rice Bran Protein
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    摘要:

    以米糠蛋白为原料,采用转谷氨酰胺酶(TG)将其改性,通过单因素试验和响应面优化试验研究蛋白质质量分数、改性时间及加酶量对改性的米糠蛋白凝胶硬度的影响,并比较改性前、后米糠蛋白的溶解性、乳化性和乳化稳定性、起泡性和起泡稳定性以及持油性等功能性质。结果表明,在蛋白质质量分数12.8%,改性时间3.2 h,加酶量19.7 U/g的条件下得到蛋白质最佳凝胶硬度,即93.58 g。经改性处理后蛋白质持水力增加162%,溶解度增加31.1%,乳化性和乳化稳定性分别提高52.7%,25.4%,起泡性和泡沫稳定性分别提高33.3%,7.2%,持油性提高114%。用扫描电子显微镜观察其微观结构,发现改性的米糠蛋白呈类似海绵结构,其结构有较明显的改善,说明酶法改性米糠蛋白效果显著。

    Abstract:

    In this paper, rice bran protein was used as raw material, and it was modified with transglutaminase (TG). The single factor experiment and response surface optimization experiment were used to study the effects of the protein mass fraction, modification time, and enzyme dosage on the protein gel hardness of modified rice bran. And the solubility, emulsifying and stabilizing properties, the foaming property, foaming stability, and oil holding property of rice bran protein before and after modification were compared. The results showed that the optimal gel hardness was obtained at a protein mass fraction of 12.8%, a modification time of 3.2 h, and an enzyme addition amount of 19.7 U/g and the gel hardness was 93.58 g. After the modification treatment, the protein water holding capacity, the solubility, emulsification and emulsification stability, foaming property and foaming stability, oil holding capacity increased by 162%, 31.1%, 52.7%, 25.4%, 33.3%, 7.2%, 114%, respectively. The microstructure of the modified rice bran protein was similar to the sponge structure observed by scanning electron microscopy. The structure of the modified rice bran protein were significantly improved, which indicated that the modified rice bran protein had a significant effect.

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于殿宇;张欣;邹丹阳;唐月;刘文质;任嘉嘉;王立琦;杜晶.酶法改性对米糠蛋白凝胶硬度及功能性质的影响[J].中国食品学报,2020,20(9):139-146

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  • 在线发布日期: 2020-10-13
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