Low-dosage glycerol monolaurate （GML） administration was able to induce dyslipidemia and low grade inflammation in mice. Lactobacillus plantarum ZJUFT34 （T34） was used to regulate the serum lipids， insulin and cytokines in overweight mice induced by GML， in order to address potential health risks associated with GML consumption. Male C57BL/6 mice were divided into two groups， fed with basic diet and basic diet containing 150 mg/kg GML， respectively. T34 was administered to mice after they exhibited significant body weight gain induced by GML. Blood samples were collected after a three-week T34 treatment. The levels of lipids， fasting glucose， insulin and cytokines in serum were determined. The results showed that the administration of T34 could maintain the significant body weight gain of the mice induced by GML and the final body weight was 2.48 g （approximately 9.3%） higher than the control group. T34 in combination with GML significantly elevated the ratio of high-density lipoprotein-cholesterol to low-density lipoprotein-cholesterol （HDL-C / LDL-C）， ameliorated insulin resistance， decreased the pro-inflammatory cytokine interleukin-6 （IL-6） level， and upregulated the anti-inflammatory cytokine transforming growth factor-β （TGF-β） level. T34 in combination with GML not only exert the beneficial effects of GML to promote mice growth， but also reduce the potential harm of GML and maintain health of mice.