两种甜叶菊废渣提取物对D-半乳糖致衰老小鼠的抗氧化作用
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北京市属高校高水平教师队伍建设支持计划(CIT&TCD201704042,IDHT20180506);国家重点研发计划项目(2018YFD0400403,2016YFD0400802);北京市科技计划项目(Z171100002217019);国家自然科学基金项目(31571801,31701575)


Antioxidant Effect of Two Kinds of Stevia Residue Extracts on D-galactose Induced Aging Mice
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    摘要:

    目的:研究两种甜叶菊废渣提取物对D-半乳糖致衰老小鼠的抗氧化作用。方法:80只小鼠随机被分为正常组、D-半乳糖组、两种甜叶菊废渣提取物低、中、高剂量组(100,200,500 mg/kg bw)。采用颈背部皮下注射D-半乳糖(500 mg/kg bw)构建衰老模型,正常组颈部皮下注射等体积的生理盐水,样品组同时给予不同剂量的甜叶菊废渣提取物。持续11周后,测定小鼠血清、肝脏和脑组织中的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)的活性和丙二醛(MDA)的含量,以及脑组织中抗氧化酶基因的相对表达量。结果:与D-半乳糖组相比,两种甜叶菊废渣提取物均在不同程度上提高小鼠血清、肝和脑抗氧化酶(SOD和GPx)的活力,降低MDA含量,且在高剂量时(500 mg/kg bw)效果最为显著。两种甜叶菊废渣提取物高剂量组小鼠脑组织中抗氧化酶基因(Nrf-2,SOD1,GPx1,HO-1)的相对表达量显著增加。结论:两种甜叶菊废渣提取物通过促进抗氧化酶基因表达,增加D-半乳糖致衰老小鼠体内相关抗氧化酶的活力并减少氧化损伤产物MDA的含量,具有潜在的延缓衰老的作用。

    Abstract:

    Objective: This article was designed to study the antioxidant activities of two kinds of stevia residue extracts on D-galactose(D-gal) induced aging mice. Methods: Eighty mice were randomly divided into control group, D-gal group, low, medium and high dose of two kinds of stevia residue extracts(100, 200, 500 mg/kg bw) groups. The aging model was established by subcutaneous injection with D-gal(500 mg/kg bw), and the mice in the control group were injected subcutaneously with the same volume of normal saline. The mice in the sample groups were given different doses of stevia residue extracts by gavage. After 11 weeks, the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), and the content of malondialdehyde (MDA) in serum, liver and brain, and the relative expression of antioxidant enzyme gene in brain tissues were measured. Results: Compared with D-gal group, two kinds of stevia residue extracts could increase the activity of serum, liver and brain antioxidant enzymes (SOD and GPx) and decrease the content of MDA in different degree, and the effect was the most obvious at high dose (500 mg/kg bw). The relative expression of antioxidant enzyme genes (Nrf-2, SOD1, GPx1, HO-1) in brain tissue of mice in the high dose group (500 mg/kg bw) was significantly increased. Conclusion: The two kinds of stevia residue extracts can increase the activity of antioxidant enzymes and decrease the MDA level in mice by promoting the gene expression level of antioxidant enzymes and possess potential anti-aging effect.

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杨华青;赵磊;徐美利;王鑫;连运河;王成涛.两种甜叶菊废渣提取物对D-半乳糖致衰老小鼠的抗氧化作用[J].中国食品学报,2020,20(10):34-42

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  • 在线发布日期: 2020-11-11
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