The toxicological properties of the new products in the pathway of sugar inhibition of PhIP was investigated by Discovery Studio（TOPKAT） toxicity prediction software， providing basic data for the comprehensive evaluation of food safety after reduction of heterocyclic aromatic amines. The results showed that the toxicity of the reaction products of α-dicarbonyl compounds with creatinine/PhIP did not change compared with PhIP in the mutagenicity （Ames test） and skin sensitization assessment. In the assessment of developmental toxicity potential （DTP）， the toxicity of the reaction product of α-dicarbonyl compound with creatinine was enhanced compared with that of PhIP， while toxicity of α-dicarbonyl compound and PhIP reaction product did not change compared with that of PhIP. In the rat oral LD50 and rat chronic oral LOAEL toxicity level assessment， the majority of the reaction products of α-dicarbonyl compounds and creatinine showed greater toxicity than PhIP， whereas toxicity of α-dicarbonyl compounds and PhIP reaction products was weaker than that of PhIP. In rat inhalation LC50 assessment， the most of the reaction products of α-dicarbonyl compounds and creatinine/PhIP showed stronger toxicity than PhIP. Thus， the formation of α-dicarbonyl compounds and creatinine/PhIP reaction products have altered their toxicity.