双歧杆菌分离株的耐药性评价
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国家自然科学基金项目(31660450);内蒙古自然科学基金项目(2017ZD07)


Antibiotic Resistance in Bifidobacterium Isolates
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    摘要:

    目的:测定20个双歧杆菌菌株对15种抗生素的最低抑菌浓度,检测菌株的耐药性基因及其是否转移。方法:采用肉汤稀释法对20株双歧杆菌菌株进行15种抗生素最小抑制浓度的检测,最终通过聚合酶链式反应检测菌株是否含有与15种抗生素相关的耐药性基因,并进一步通过滤膜杂交法测定耐药性基因是否转移。结果:在双歧杆菌分离株之间,最小抑菌浓度有显著差异;同一种的双歧杆菌最小抑菌浓度也不尽相同。所有双歧杆菌菌株对氨苄西林、庆大霉素、链霉素、利奈唑胺敏感,对卡那霉素有内在抗性;各菌株对氯霉素、环丙沙星、克林霉素、红霉素、新霉素、四环素、甲氧苄啶、万古霉素的敏感性存在差异。聚合酶链式反应分析表明,部分双歧杆菌中含有tet(W)。然而,滤膜杂交试验表明:含有抗性基因的菌株,抗性基因并不会发生转移,菌株具有安全性。结论:双歧杆菌与抗生素耐药性细菌无关,20株双歧杆菌是安全菌株。

    Abstract:

    Objective: The minimum inhibitory concentrations (MICs) for 15 antibiotics of 20 Bifidobacterium isolates were determined and the transfer of antibiotic-resistance genes was assayed. Methods: 20 strains of Bifidobacterium strains were tested for the minimum inhibitory concentration of 15 antibiotics by broth dilution method, and the specific primers were used to detect whether the strain contained drug resistance genes by polymerase chain reaction. Finally, the drug resistance gene transferred was tested by filter hybridization. Results: The MIC values varied significantly among the Bifidobacterium isolates, including those of the same species. All of the Bifidobacterium isolates were susceptible to ampicillin, gentamicin, streptomycin, and linezolid and intrinsically resistant to kanamycin; in contrast, the susceptibility to chloramphenicol, ciprofloxacin, clindamycin, erythromycin, neomycin, tetracycline, trimethoprim, and vancomycin varied among the isolates. PCR analysis showed that several Bifidobacterium isolates harbored tet(W). However, filter-mating experiments indicated no transfer of antibiotic-resistance genes. Conclusion: Bifidobacteria are not involved in further emergence of antibiotic resistant bacteria and that they are safe enough in this issue.

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潘琳;郭慧玲;李丽娜;张文羿;陈永福;孟和毕力格.双歧杆菌分离株的耐药性评价[J].中国食品学报,2020,20(11):218-227

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  • 在线发布日期: 2020-12-04
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