基于Caco-2细胞模型的3种大豆肽降胆固醇能力研究
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金项目(31871839); 北京优秀人才培养资助青年拔尖人才计划项目(CIT&TCD201904038)


Studies on the Cholesterol Lowering Ability of Three Soybean Peptides in Caco-2 Cells Model
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    为了探究具有降胆固醇生物活性的大豆多肽在机体内的转运吸收机制,本研究以3种降胆固醇大豆肽VAWWMY(VY-6)、LPYPR(LR-5)、WGAPSL(WL-6)为研究对象,以22-NBD胆固醇胶束孵育的Caco-2细胞模型为高胆固醇模型,探究这3种多肽抑制胆固醇溶解,影响细胞吸收胆固醇与抑制胆固醇正向转运的能力。研究结果表明,在抑制胆固醇溶解率方面:WL-6多肽浓度为0.5 mmol/L时,在3种多肽中的抑制率最高,达14.2%,之后随浓度升高抑制率降低;在抑制细胞对胆固醇吸收方面:VY-6多肽浓度为2 mmol/L时,抑制率最大为43.1%,LR-5多肽浓度为2.5 mmol/L时,抑制率最大为45.7%,WL-6多肽浓度为2 mmol/L时,抑制率最大为45.93%,WL-6多肽在3种大豆肽中的抑制能力最强,且高于辛伐他汀组;在抑制胆固醇转运能力方面:WL-6多肽浓度为2 mmol/L时,可抑制30.7%的胆固醇转运,抑制率最高。综上可知WL-6多肽在3种大豆多肽中的降胆固醇能力最强。

    Abstract:

    In order to explore the absorption mechanism of soybean peptides with the biological activity, 22-NBD cholesterol micelle incubated Caco-2 cells were used as a high cholesterol model. Effects of three kinds of soybean peptides VAWWMY(VY - 6), LPYPR (LR - 5), and WGAPSL (WL - 6) on the cholesterol dissolution inhibition rate, absorb cholesterol capacity, and cholesterol transport were investigated. Results indicated that WL-6 had the highest cholesterol dissolution inhibition rate at 0.5 mmol/L concentration. However, the inhibition rate gradually decreased with the increasing concentrations. WL-6 could inhibit 14.2% of cholesterol uptaking by cells at the concentration of 2 mmol/L. For the cholesterol absorbing capacity, VY-6 and WL-6 had the best inhibition rate of 43.1% and 45.95% at the concentration of 2 mmol/L, respectively. LR-5 had the best inhibition absorbing rate of 45.7% at the concentration of 2.5 mmol/L, which indicated that WL-6 had the best cholesterol absorbing capacity and higher than simvastatin. In the experiment of cholesterol transport, WL-6 had the best inhibition rate of 30.7% at the concentration of 2 mmol/L. Above all, WL-6 had the best power at cholesterol lowering effect.

    参考文献
    相似文献
    引证文献
引用本文

张慧娟;付冰冰;王静;.基于Caco-2细胞模型的3种大豆肽降胆固醇能力研究[J].中国食品学报,2021,21(1):44-50

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2021-02-22
  • 出版日期:
版权所有 :《中国食品学报》杂志社     京ICP备09084417号-4
地址 :北京市海淀区阜成路北三街8号9层      邮政编码 :100048
电话 :010-65223596 65265375      电子邮箱 :chinaspxb@vip.163.com
技术支持:北京勤云科技发展有限公司

漂浮通知