基于靶向蛋白质组学的乳制品中牛血清白蛋白的定量检测方法
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Quantitative Determination Method for Bovine Serum Albumin in Milk Products Based on Targeted Proteomics
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    摘要:

    基于靶向蛋白质组学技术,使用高分辨质谱(Q-Orbitrap)和蛋白软件对理论肽段进行定性确认,根据肽段特异性、酶解曲线和稳定性结合其它肽段筛选策略确定定量特征肽段,建立乳制品(婴幼儿配方乳粉和乳清粉)中牛血清白蛋白的超高效液相色谱-串联质谱检测方法。该方法特异性强,两肽段检出限都低于1 mg/100 g,回收率在87.9%~101.1%之间,日内、日间精密度均小于7.6%。对12份婴幼儿配方乳粉和乳清粉的检测结果表明,该方法适用于乳制品中牛血清白蛋白的定量检测,为婴幼儿配方乳粉的质量监控提供了技术支持。

    Abstract:

    The ultra performance liquid chromatography-tandem mass spectrometry method was established to quantify the bovine serum albumin in bovine dairy products accurately based on targeted proteomics. Qualitative analysis of theoretical peptides was carried out using high-resolution mass spectrometry (Q-Orbitrap) and protein software. Based on the the specificity and stability of peptides and the dynamics curve of enzymatic hydrolysis, LVNELTEFAK and HLVDEPQNLIK were selected as the specific proteotypic peptides for quantitative determination of bovine serum albumin. The detection limits of both peptides were less than 1 mg/100 g. And the recovery rates were between 87.9% and 101.1%, the intra-day and the inter-day precisions were less than 7.6%. The detection of 12 infant formula and whey powder showed that the method was suitable for the quantitative detection of bovine serum albumin in dairy products. It provides a technical foundation for quality monitoring of infant formula.

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周荧;陈雨湉;赖世云;胡蓓;任一平;.基于靶向蛋白质组学的乳制品中牛血清白蛋白的定量检测方法[J].中国食品学报,2021,21(1):256-265

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  • 在线发布日期: 2021-02-22
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