高吸附铅乳酸菌的微胶囊化及特性
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内蒙古自治区成果转化项目(CGZH2018145)


Microencapsulation and Its Characteristic of Lactic Acid Bacteria with Highly Adsorbed Ability of Lead
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    摘要:

    乳酸菌作为人体重要的微生物之一,在肠道内发挥益生作用,然而,其不良的环境耐受能力,使其生长、定植受到影响。研究乳酸菌的微胶囊化对提高其环境耐受性具有重要的意义。本研究选取具有高耐受和吸附铅能力的戊糖片球菌10-a-1为研究对象,采用内源乳化法制备海藻酸钠微胶囊,并优化其工艺。通过单因素试验和正交试验确定最优工艺参数,在此基础上利用壳聚糖进行二次包埋,比较两种微胶囊的特性。结果表明:在海藻酸钠质量分数3%,水油质量比30 ∶ 120,酸钙质量比3 ∶ 1,转速600 r/min,钙胶质量比1.5 ∶ 9条件下的海藻酸钠微胶囊的包埋率最高,可达86.3%。海藻酸钠-壳聚糖微胶囊的包埋率为65.6%。对比两种微胶囊的特性,海藻酸钠微胶囊比海藻酸钠-壳聚糖微胶囊释放快;海藻酸钠-壳聚糖微胶囊耐胃肠液的效果好,存活率高;两种微胶囊均可增强模拟胃肠液处理中菌吸附铅的能力,且海藻酸钠-壳聚糖微胶囊对铅的吸附效果更好。在4 °C和20 °C条件下分别贮藏28 d,海藻酸钠-壳聚糖微胶囊中菌的存活率最高。微胶囊化技术可显著提高戊糖片球菌10-a-1对不良环境的耐受能力,并减小模拟胃肠液处理对戊糖片球菌10-a-1吸附铅能力的影响。

    Abstract:

    As one of the important microorganisms in the human body, lactic acid bacteria (LAB) play a probiotic role in the intestine. However, the growth and colonization of LAB are affected by its poor environmental tolerance. Therefore, it is important to explore the microencapsulation of LAB to improve its environmental tolerance. The strain of Pediococcus pentosaceus 10-a-1 with high tolerance and adsorption capacity of lead was used in the research. Sodium alginate microcapsules were prepared by internal emulsification and the technological conditions were optimized. The optimum technological parameters were determined by single factor test and orthogonal test. On this basis, chitosan was used for secondary embedding. The characteristics of the two microcapsules were compared. The results showed optimum process conditions were determined, sodium alginate concentration was 3%, water-oil ratio at 30∶120, calcium acid ratio at 3∶1, stirring speed was 600 r/min, the ratio of calcium and gel was 1.5∶9. Under the optimized conditions, the encapsulation rate of the sodium alginate microcapsules reached 86.3%. After the encapsulation of sodium alginate microcapsules by chitosan, the embedding rate of microcapsules decreased to 65.6%. The characteristics of the two microcapsules was compared, sodium alginate microcapsules released more quickly than sodium alginate-chitosan microcapsules. The sodium alginate- chitosan microcapsules showed better tolerance to gastric and intestinal fluids and resulted in the higher survival rate. The adsorption capacity of lead in sodium alginate-chitosan microcapsules was better. The survival rate of LAB in sodium alginate-chitosan microcapsules was the highest after 28 days of storage at 4 ℃ and 20 ℃, respectively. The microencapsulation technology could significantly improve the tolerance of P. pentosaceus 10-a-1 to adverse environment. Meanwhile, the effect of simulated gastrointestinal fluid treatment on the ability of P. pentosaceus 10-a-1 to adsorb lead could be reduced.

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董阳;贺佳鑫;顾悦;贺银凤.高吸附铅乳酸菌的微胶囊化及特性[J].中国食品学报,2021,21(3):173-182

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  • 在线发布日期: 2021-04-19
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