冷休克处理提高嗜酸乳杆菌的冻干存活率
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国家自然科学基金项目(32072195,41406165,41641052);浙江省公益技术研究计划(LGN19C 200011);宁波市公益性计划(202002N3068)


Improvement of Freeze-drying Survival Rate of Lactobacillus acidophilus by Cold Shock Treatme
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    摘要:

    冷冻干燥法为制备直投式发酵剂常用的方法,然而,在冻干过程中,菌的存活率会降低而影响发酵剂的活性。为提高嗜酸乳杆菌冷冻干燥后的存活率,本试验通过单因素和响应面试验对冷冻干燥前的嗜酸乳杆菌进行冷休克处理条件优化。结果表明:最佳冷休克处理条件是:在37 ℃培养10 h时收集嗜酸乳杆菌,8 ℃冷休克处理15 h,再结合复合冻干保护剂,使其冷冻干燥后的存活率提高至96.64%;比未冷休克、添加冻干保护剂的P2组(78.99%)高17.65%,比未冷休克、未添加冻干保护剂的P4组(21.42%)高75.22%。这说明冷冻干燥前对乳酸菌进行冷休克处理是提高其抗冻干胁迫能力的一种有效新途径。

    Abstract:

    The freeze-drying method is commonly used for preparing directed vas set (DVS). However, in the process of freeze-drying, the survival rate of bacteria decreases and the activity of starter is affected. In order to improve the survival rate of Lactobacillus acidophilus after freeze-drying, the cold shock treatment of L. acidophilus before freeze-drying was optimized by single factor experiments and response tests. The optimal cold shock treatment conditions were as follows. L. acidophilus were incubated in MRS broth at 37 ℃ for 10 hours, and then be treated by cold shock treatment at 8 ℃ for 15 hours combing with composite cryoprotectant. The survival rate of L.acidophilus after freeze-drying increased to 96.64% which was higher than the group adding cryoprotectant but without cold shock treatment(78.99%) as well as the group without cold shock and no cryoprotectant (21.42%), the survival rates increased 17.65%, 75.22% respectively. The results indicate of that cold shock treatment before freeze-drying is a new effective way to improve the tolerance of lactic acid bacteria to freeze-drying stress and increasing the survival rate of lactic acid bacteria for preparation of high-activity DVS.

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王晓萌;甄妮;田启远;叶聪艳;曾小群;潘道东;陈小虎.冷休克处理提高嗜酸乳杆菌的冻干存活率[J].中国食品学报,2021,21(3):203-209

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  • 在线发布日期: 2021-04-19
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