Quorum sensing gene luxI/luxR of Aeromonas sobria was used for this study， and the effect of different environmental conditions on the expression of this gene was investigated from the transcription level by real-time fluorescence quantitative PCR to determine the regulatory effect of environmental factors on quorum sensing system. The results showed that the expression level of luxI/luxR gene was the highest at appropriate low temperature （15 ℃） and weak base （pH = 8）， while the expression level of both genes decreased under high osmotic stress. When glucose was the carbon source， the expression of luxI/luxR gene was the highest， and luxI gene was more sensitive to carbon sources than luxR gene. The expression level of luxI gene under different nitrogen sources was as follows： tryptone > ammonium chloride > beef extract > yeast extract > urea， and the expression of luxR gene was as follows： tryptone > ammonium chloride > yeast extract > beef extract > urea. Therefore， the changes of environmental conditions can be used to regulate quorum sensing system in Aeromonas sobria， so as to control its spoilage ability.