The changes of biogenic amines in Illexargentinus were determined by high performance liquid chromatography （HPLC） at different storage temperatures. The main characteristics of biogenic amines were determined and their freshness was evaluated. The amine-producing bacteria were isolated and identified. The results showed that the main biogenic amines produced in Illexargentinus were phenethylamine， putrescine， cadaverine and tyramine. Histamine， spermine and spermidine did not change significantly during storage. The TBA and TCA indexes in Illexargentinusat at different storage temperatures with the prolongation of storage time were increasing， while the pH value decreased first and then increased. Amine strains A1-A11 and B1-B12 were isolated under storage conditions of 25 ℃ and 4 ℃， among which 16 strains of saprophyte， 16 strains of cadaverine， 14 strains of histamine and 2 strains of tyramine were isolated. 5 strains （Citrobacter freundii， Citrobacter murliniae， Citrobacter braakii）， 2 strains （Hafnia alvei）， 1 strain （Pantoea agglomerans）， 1 strain （Obesumbacterium proteus）， 1 strain （Kluyvera cryocrescens） and 1 strain （Klebsiella aerogenes） were screened under the condition of 25 ℃. 1 strain of Enterobacteriaceae bacterium， 1 strain of Citrobacter braakii， 1 strain of Klebsiella aerogenes， 3 strains of Obesumbacterium proteus， 6 strains （Hafnia alvei， Hafnia alvei sensu， Hafnia paralvei） were screened under the storage condition of 4 ℃.