甜叶菊废渣提取物及其主要成分异绿原酸的抗炎作用
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“十三五”国家重点研发计划项目(2018YFD 0400403,2016YFD0400802);北京市科技计划项目(Z171100002217019);国家自然科学基金项目(31571801,31701575)


Anti-inflammatory Effect of Stevia Residue Extract and Its Main Components Isochlorogenic Acids
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    摘要:

    目的:研究甜叶菊废渣提取物及其主要成分异绿原酸的抗炎作用。方法:通过建立LPS诱导小鼠单核巨噬细胞(RAW264.7)炎症模型和角叉菜胶致小鼠足部肿胀模型,以一氧化氮(NO)、前列腺素E2(PGE2)、超氧化物歧化酶(SOD)和丙二醛(MDA)等为检测指标,检测甜叶菊废渣提取物及其主要成分异绿原酸的体内外抗炎作用。结果:体外RAW264.7细胞炎症模型结果显示,甜叶菊废渣提取物及其主要成分异绿原酸均能抑制RAW264.7细胞产生NO,抗炎效果为异绿原酸C≈甜叶菊废渣提取物>异绿原酸A。3种样品对小鼠足肿胀有显著的抑制作用,抑制效果为异绿原酸C>异绿原酸A≈甜叶菊废渣提取物。高剂量的甜叶菊废渣提取物及其主要成分异绿原酸能抑制小鼠肝脏NO的生成,甜叶菊废渣提取物和异绿原酸C可显著降低小鼠血清NO和PGE2含量,且异绿原酸A与异绿原酸C能显著提高小鼠SOD活性并降低MDA含量,减轻机体自由基损伤。3种样品对小鼠肝脏PGE2生成无显著影响。结论:甜叶菊废渣提取物中的异绿原酸类物质具有很好的抗炎效果。

    Abstract:

    Objective: To study the anti-inflammatory effects of the stevia residue extract and its main components-isochlorogenic acids. Methods: Nitric oxide (NO), prostaglandin E2 (PGE2), superoxide dismutase (SOD) and malondialdehyde (MDA) were used to study the anti-inflammatory effects of the stevia residue extract and its main components-isochlorogenic acids both in vitro and in vivo by establishing LPS-induced RAW264.7 cell inflammation model and carrageenan-induced mouse paw edema model. Results: In vitro RAW264.7 cell inflammation model showed that the extract of stevia residue extract and its main components was ochlorogenic acids which could inhibit the production of NO in inflammatory cells. The anti-inflammatory effects were in the order: isochlorogenic acid C ≈ stevia sulphate extract > isochlorogenic acid A. The three samples had significant inhibitory effects on mouse paw edema. The inhibitory effects were in the order: isochlorogenic acid C > isochlorogenic acid A ≈ stevia residue extract. At high dose, the stevia residue extract and its main components-isochlorogenic acids, inhibited the production of NO in the liver of mice. Stevia residue extract and isochlorogenic acid C could significantly reduce serum NO and PGE2 levels in mice;and isochlorogenic acid A and isochlorogenic acid C could significantly increase SOD activity and reduce MDA content in mice, leading to reduced degree of body damage attacked by free radicals. All the three samples had no significant effect on PGE2 production in mice. In summary, the isochlorogenic acids in the stevia residue extract have a good anti-inflammatory effect.

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赵磊;张会敏;徐美利;鲍玺;艾欣;陈艳麟;王成涛;连运河.甜叶菊废渣提取物及其主要成分异绿原酸的抗炎作用[J].中国食品学报,2021,21(5):117-124

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  • 在线发布日期: 2021-06-07
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