The study investigated the in vitro fermentation of polysaccharide from Fagopyrum esculentum moench bee pollen（WFPP）， the effects of WFPP on the metabolites produced by human intestinal flora. By establishing an in vitro fermentation model， the pH value， total sugar content， NH3-N content and SCFAs content of fermentation broth were determined. The fermentation behavior of WFPP was analyzed to explore the beneficial effects of WFPP on human intestinal flora. The pH value and content of total sugar in the fecal culture declined as fermentation time increased， after 24 h of fermentation， the pH value of WFPP-H group decreased from 7.26 to 4.64， the percentage of the total carbohydrates consumed in WFPP groups reached up to 80%. The accumulation of NH3-N content was reduced significantly by adding WFPP in culture broth， and the higher the content of WFPP， the better the effect. Acetic， propionic and N-butyric acid were the dominant short-chain fatty acids （SCFAs）， which got a significantly rise in fermentation， the content of SCFAs in the culture broth of polysaccharide groups was WFPP-H>WFPP-M>WFPP-L. The results showed that WFPP could be hydrolyzed be human intestinal flora， which produced SCFAs lowered the pH value， reduced the accumulation of NH3-N， the WFPP have a potential prebiotic effect.