液相色谱-串联质谱同时测定食用植物油中的多种真菌毒素
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国家重点研发计划项目(2017YFC1601603)


Simultaneous Detection of Multi-mycotoxins in Edible Oil Using Liquid Chromatography Tandem Mass Spectrometry
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    摘要:

    基于分步液液提取以及脂质净化柱净化的样品前处理方式,建立同时测定食用植物油中28种真菌毒素的高效液相色谱-串联质谱的测定方法。28种真菌毒素的定量限范围0.07~1.25 μg/kg,三水平加标回收率范围60%~120%,峰面积相对标准偏差小于12.5%,均满足国家标准GB/T 27404-2008《实验室质量控制规范 食品理化检测》中实验室质量控制规范以及真菌毒素限量标准的要求。应用本方法对市售食用植物油样品进行真菌毒素的风险监测,结果显示:黄曲霉毒素、玉米赤霉烯酮及其衍生物、杂色曲霉素、T-2毒素有不同程度的检出。其中,花生油中主要的真菌毒素种类为黄曲霉毒素、杂色曲霉素以及玉米赤霉烯酮;菜籽油和玉米油中主要的真菌毒素种类为玉米赤霉烯酮及其衍生物。

    Abstract:

    A method for simultaneous detection of 28 mycotoxins in edible oil was established by ultra performance liquid chromatography-tandem mass spectrometry. Sample preparation was fulfilled through two-step liquid-liquid extraction and solid-phase extraction sequentially. The methodological verification results showed that the limits of quantification (S/N, 10) ranged from 0.07 μg/kg to 1.25 μg/kg, which were far less than the maximum levels of mycotoxins in foods. In addition, the recoveries of the three-level spiked samples were between 60% and 120%, and the relative standard deviations were less than 12.5%, which could meet the quality control specifications required in the national standard GB/T 27404-2008 Criterion on quality control of laboratories - Chemical testing of food. Using this method, risk monitoring of mycotoxins in the edible oils was executed, showing that aflatoxins, zearalenones and derivatives, sterigmatocysin and T-2 could possibly contaminated edible oils. In peanut oils, aflatoxins, zearalenone and sterigmatocysin were the main mycotoxins while zearalenone and its derivatives were the main mycotoxins in both rapeseed oils and corn oils.

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杨帅;杨永坛;穆蕾;杨霞;杨悠悠;罗云敬.液相色谱-串联质谱同时测定食用植物油中的多种真菌毒素[J].中国食品学报,2021,21(5):309-316

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  • 在线发布日期: 2021-06-07
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