不同分子质量ε-聚-L-赖氨酸对金黄色葡萄球菌的抑菌机制
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国家重点研发计划项目(2018YFD0400205); 国家自然科学基金项目(31771952)


Antibacterial Mechanism of ε-Poly-L-lysine with Different Molecular Weight on Staphylococcus aureus
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    摘要:

    不同分子质量的ε-PL具有不同的抑菌活性,而造成这种差异性的机制尚不清晰。本研究选用革兰氏阳性菌金黄色葡萄球菌作为研究对象,利用扫描电子显微镜(SEM)、酶标仪、气相色谱-质谱联用仪(GC-MS)等方法,从抑菌活性大小、代谢水平差异和细胞壁变化等方面,研究了不同分子质量ε-PL引起的抑菌差异,探讨了不同分子质量ε-PL对金黄色葡萄球菌的作用机制。结果表明,与低分子质量(3 ku)能使细胞表面出现不同程度的凹陷和皱缩。同时利用代谢组学的方法,分析了不同分子质量ε-PL对金黄色葡萄球菌在代谢水平上的抑菌差异,发现较高分子质量(1~3 ku和>3 ku)ε-PL和ε-PL产品对糖酵解途径和三羧酸循环的抑制作用远大于低分子质量(

    Abstract:

    Different molecular weights of ε-PL have different antibacterial activities, but the mechanism that causes this difference is not clear. In this study, Gram-positive bacteria Staphylococcus aureus was selected as the research object. From the aspects of antibacterial activity, metabolic level, and cell wall, scanning electron microscope (SEM), microplate reader, gas chromatography-mass spectrometry (GC-MS) and other instruments analyzed the difference in bacteriostatic activity caused by different molecular weight ε-PL, and explored the mechanism of different molecular weight ε-PL on S. aureus. The experiment results showed that compared with the low-molecular-weight(<1 ku) ε-PL treatment group, the high-molecular-weight ε-PL(1-3 ku and >3 ku) makes the surface of the cells sag and shrink to different degrees. The metabolomics method was used to study the differences in the metabolic levels of S. aureus after the action of different molecular weight ε-PL. The results showed that high molecular weight (1-3 ku and > 3 ku) ε-PL and the initial ε-PL inhibited the glycolytic pathway and tricarboxylic acid cycle much more than the low molecular weight (<1 ku) ε-PL. It has been verified that the bacteriostatic effects to S. aureus with different molecular weight ε-PL are different, which provides theoretical support for the use of preservatives in actual food processing and production processes.

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廖钰婷;王贺莉;董天宇;杨萍;唐昆鹏;谭之磊;贾士儒.不同分子质量ε-聚-L-赖氨酸对金黄色葡萄球菌的抑菌机制[J].中国食品学报,2021,21(7):28-36

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  • 在线发布日期: 2021-08-12
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